Int J Oral Biol.  2019 Sep;44(3):96-100. 10.11620/IJOB.2019.44.3.96.

Peptoniphilus mikwangii-specific quantitative real-time polymerase chain reaction primers

Affiliations
  • 1Korean Collection for Oral Microbiology and Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwangju 61452, Republic of Korea. jkkook@chosun.ac.kr

Abstract

The purpose
of this study was to develop Peptoniphilus mikwangii -specific quantitative real-time polymerase chain reaction (qPCR) primers based on the 16S ribosomal RNA (16S rDNA) gene. The specificity of the primers was determined by conventional PCR using 29 strains of 27 oral bacterial species including P. mikwangii. The sensitivity of the primers was determined by qPCR using the purified genomic DNA of P. mikwangii KCOM 1628(T) (40 ng to 4 fg). The data showed that the qPCR primers (RTB134-F4/RTB134-R4) could detect P. mikwangii strains exclusively and as little as 40 fg of the genomic DNA of P. mikwangii KCOM 1628(T). These results suggest that the developed qPCR primer pair can be useful for detecting P. mikwangii in epidemiological studies of oral bacterial infectious diseases.

Keyword

Peptoniphilus mikwangii; 16S rDNA; qPCR primers
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