J Lab Med Qual Assur.  2011 Jun;33(1):43-47.

Evaluation of two Real-Time PCR Assays for the Detection of Chlamydia trachomatis

Affiliations
  • 1Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea. sykim@yuhs.ac
  • 2Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea.

Abstract

BACKGROUND
Chlamydia trachomatis is common etiological agent of sexual transmitted disease. We evaluated two real-time PCR assays for the detection of C. trachomatis; Cobas TaqMan CT test v2.0 (Roche Diagnostics, Switzerland) and AdvanSure CT/NG real-time PCR (LG Life Sciences, Korea). And we compared performance of two real-time PCR assays with Cobas Amplicor CT test (Roche Diagnostics, Switzerland).
METHODS
Seventy-nine cervical swab specimens collected from patients with suspected C. trachomatis infection were tested using CTM CT, ADVANSURE, and AMPLICOR for the detection of C. trachomatis.
RESULTS
Twenty-five (31.6%) samples showed positive results in all three assays. The positive and negative concordance rates among three assays were 100%.
CONCLUSIONS
CTM CT and ADVANSURE showed excellent agreement with AMPLICOR. Therefore, these assays were reliable for the evaluation of C. trachomatis infection.

Keyword

Chlamydia trachomatis; Real-time polymerase chain reaction; Comparison
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