J Korean Acad Prosthodont.  2020 Jan;58(1):14-22. 10.4047/jkap.2020.58.1.14.

The biofilm removal effect of MnOâ‚‚-diatom microbubbler from the dental prosthetic surfaces: In vitro study

Affiliations
  • 1Department of Prosthodontics, School of Dentistry, Seoul National University, Seoul, Republic of Korea. silk1@snu.ac.kr
  • 2Department of Chemical and Biomolecular Engineering, University of Illinois, Urbana-Champaign, Urbana, IL, USA.

Abstract

PURPOSE
The aim of this study is to evaluate the effectiveness of MnOâ‚‚-diatom microbubbler (DM) on the surface of prosthetic materials as a mouthwash by comparing the biofilm removal effect with those previously used as a mouthwash in dental clinic.
MATERIALS AND METHODS
DM was fabricated by doping manganese dioxide nanosheets to the diatom cylinder surface. Scanning electron microscopy (SEM) was used to observe the morphology of DM and to analyze the composition of doped MnOâ‚‚. Stereomicroscope was used to observe the reaction of DM in 3% hydrogen peroxide. Non-precious metal alloys, zirconia and resin specimens were prepared to evaluate the effect of biofilm removal on the surface of prosthetic materials. And then Streptococcus mutans and Porphyromonas gingivalis biofilms were formed on the specimens. When 3% hydrogen peroxide solution and DM were treated on the biofilms, the decontamination effect was compared with chlorhexidine gluconate and 3% hydrogen peroxide solution by crystal violet staining.
RESULTS
Manganese dioxide was found on the surface of the diatom cylinder, and it was found to produce bubble of oxygen gas when added to 3% hydrogen peroxide. For all materials used in the experiments, biofilms of the DM-treated groups got effectively removed compared to the groups used with chlorhexidine gluconate or 3% hydrogen peroxide alone.
CONCLUSION
MnOâ‚‚-diatom microbubbler can remove bacterial membranes on the surface of prosthetic materials more effectively than conventional mouthwashes.

Keyword

Dental plaque; Hydrogen peroxide; MnOâ‚‚-diatom microbubbler; Mouthwash; Oral hygiene

MeSH Terms

Alloys
Biofilms*
Chlorhexidine
Decontamination
Dental Clinics
Dental Plaque
Diatoms
Gentian Violet
Hydrogen Peroxide
In Vitro Techniques*
Manganese
Membranes
Microscopy, Electron, Scanning
Mouthwashes
Oral Hygiene
Oxygen
Porphyromonas gingivalis
Streptococcus mutans
Alloys
Chlorhexidine
Gentian Violet
Hydrogen Peroxide
Manganese
Mouthwashes
Oxygen
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