Biomol Ther.  2019 Sep;27(5):457-465. 10.4062/biomolther.2018.214.

Enrichment of Short-Chain Ceramides and Free Fatty Acids in the Skin Epidermis, Liver, and Kidneys of db/db Mice, a Type 2 Diabetes Mellitus Model

Affiliations
  • 1Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of Korea. kmlim@ewha.ac.kr, hha@ewha.ac.kr
  • 2Severance Biomedical Science Institute, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. kitaek@yuhs.ac
  • 3Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Republic of Korea.

Abstract

Patients with diabetes mellitus (DM) often suffer from diverse skin disorders, which might be attributable to skin barrier dysfunction. To explore the role of lipid alterations in the epidermis in DM skin disorders, we quantitated 49 lipids (34 ceramides, 14 free fatty acids (FFAs), and cholesterol) in the skin epidermis, liver, and kidneys of db/db mice, a Type 2 DM model, using UPLC-MS/MS. The expression of genes involved in lipid synthesis was also evaluated. With the full establishment of hyperglycemia at the age of 20 weeks, remarkable lipid enrichment was noted in the skin of the db/db mice, especially at the epidermis and subcutaneous fat bed. Prominent increases in the ceramides and FFAs (>3 fold) with short or medium chains (

Keyword

Lipids; Ceramide; Free fatty acid; Diabetes mellitus; Skin barrier function

MeSH Terms

Animals
Ceramides*
Diabetes Mellitus
Diabetes Mellitus, Type 2*
Epidermis*
Fatty Acids, Nonesterified*
Humans
Hyperglycemia
Kidney*
Liver*
Mice*
Receptors, Cytoplasmic and Nuclear
Skin*
Stearoyl-CoA Desaturase
Subcutaneous Fat
Ceramides
Fatty Acids, Nonesterified
Receptors, Cytoplasmic and Nuclear
Stearoyl-CoA Desaturase
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