Endocrinol Metab.  2019 Jun;34(2):203-212. 10.3803/EnM.2019.34.2.203.

Expression of NF2 Modulates the Progression of BRAFV600E Mutated Thyroid Cancer Cells

Affiliations
  • 1Division of Endocrinology and Metabolism, Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. wongukim@amc.seoul.kr

Abstract

BACKGROUND
We previously reported the frequent neurofibromatosis 2 (NF2) gene mutations in anaplastic thyroid cancers in association with the BRAF V600E mutation. We aimed to investigate the role of NF2 in thyroid cancer with BRAF mutation.
METHODS
To identify the function of NF2 in thyroid cancers, we investigated the changes in cell proliferation, colon formation, migration and invasion of thyroid cancer cells (8505C, BHT101, and KTC-1) with BRAF V600E mutation after overexpression and knock-down of NF2. We also examined how cell proliferation changed when NF2 was mutagenized. Human NF2 expression in papillary thyroid carcinoma (PTC) was analyzed using the The Cancer Genome Atlas (TCGA) data.
RESULTS
First, NF2 was overexpressed in 8505C and KTC-1 cells. Compared to control, NF2 overexpressed group of both thyroid cancer cells showed significant inhibition in cell proliferation and colony formation. These results were also confirmed by cell migration and invasion assay. After knock-down of NF2 in 8505C cells, there were no significant changes in cell proliferation and colony formation, compared with the control group. However, after mutagenized S288* and Q470* sites of NF2 gene, the cell proliferation increased compared to NF2 overexpression group. In the analysis of TCGA data, the mRNA expression of NF2 was significantly decreased in PTCs with lateral cervical lymph node (LN) metastasis compared with PTCs without LN metastasis.
CONCLUSION
Our study suggests that NF2 might play a role as a tumor suppressor in thyroid cancer with BRAF mutation. More studies are needed to elucidate the mechanism how NF2 acts in thyroid cancer with BRAF mutation.

Keyword

Neurofibromatosis 2; BRAF, mutation; Thyroid neoplasms; Genes, tumor suppressor

MeSH Terms

Cell Movement
Cell Proliferation
Colon
Genes, Neurofibromatosis 2
Genes, Tumor Suppressor
Genome
Humans
Lymph Nodes
Neoplasm Metastasis
Neurofibromatosis 2
RNA, Messenger
Thyroid Carcinoma, Anaplastic
Thyroid Gland*
Thyroid Neoplasms*
RNA, Messenger
Full Text Links
  • ENM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error