Ann Dermatol.  2019 Apr;31(2):154-163. 10.5021/ad.2019.31.2.154.

Neuropeptides Profile and Increased Innervation in Becker's Nevus

Affiliations
  • 1Department of Dermatology, Dankook University College of Medicine, Cheonan, Korea. ivymyung@hanmail.net

Abstract

BACKGROUND
Melanocytes are derived from neural crest, and various pigmentary disorders may accompany abnormalities in nerve system or develop following dermatome, suggesting that melanocyte and pigmentation may be closely related to neural factors. There are reports of Becker's nevus (BN) showing linear and segmental configuration, suggesting the association of BN with nerve system. However, there are no studies regarding the expression of neuropeptides in BN.
OBJECTIVE
We investigated the expression of neuropeptides and innervation in BN.
METHODS
Polymerase chain reaction (PCR) array of 84 genes related to neuronal process was done. Among the genes with 10-fold or more increase in lesional, real-time PCR was performed for neuropeptide Y (NPY), galanin, neurotensin (NTS) and their receptors skin compared to normal skin. IHC stain was done to look for the expression of NPY, galanin, NTS and their receptors and the distribution of protein gene products (PGP) 9.5 immunoreactive nerve fibers.
RESULTS
PCR array revealed that 16 out of 84 genes related to neuronal process were increased by 10-fold or more in lesional skin. In real-time PCR of NPY, galanin, NTS and their receptors, statistically significant increase of NPY1R (p < 0.05) and marginally significant increase of NPY2R, GAL2R, and NTS2R (p < 0.1) was verified in lesional skin. In immunohistochemistry, NPY, NPY1R NPY2R, and NTS2R were highly expressed in lesional skin and increased PGP 9.5 immunoreactive linear nerve fibers were found in the epidermis of BN.
CONCLUSION
NPY, galanin, NTS and their receptors and increased innervation may play a role in the pathogenesis of BN.

Keyword

Becker nevus; Galanin; Neuropeptide Y; Neurotensin; Protein gene product 9.5

MeSH Terms

Epidermis
Galanin
Immunohistochemistry
Melanocytes
Nerve Fibers
Neural Crest
Neurons
Neuropeptide Y
Neuropeptides*
Neurotensin
Nevus*
Pigmentation
Polymerase Chain Reaction
Proteins
Real-Time Polymerase Chain Reaction
Skin
Galanin
Neuropeptide Y
Neuropeptides
Neurotensin
Proteins
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