Korean J Physiol Pharmacol.  2018 Nov;22(6):671-677. 10.4196/kjpp.2018.22.6.671.

Tussilagone suppressed the production and gene expression of MUC5AC mucin via regulating nuclear factor-kappa B signaling pathway in airway epithelial cells

Affiliations
  • 1Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 35015, Korea. LCJ123@cnu.ac.kr
  • 2Smith Liberal Arts College and Department of Addiction Science, Graduate School, Sahmyook University, Seoul 01795, Korea. hjy1213@syu.ac.kr

Abstract

In the present study, we investigated whether tussilagone, a natural product derived from Tussilago farfara, significantly affects the production and gene expression of airway MUC5AC mucin. Confluent NCI-H292 cells were pretreated with tussilagone for 30 min and then stimulated with EGF (epidermal growth factor) or PMA (phorbol 12-myristate 13-acetate) for 24 h or the indicated periods. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. To elucidate the action mechanism of tussilagone, effect of tussilagone on PMA-induced NF-κB signaling pathway was investigated by western blot analysis. Tussilagone significantly inhibited the production of MUC5AC mucin protein and down-regulated the expression of MUC5AC mucin gene, induced by EGF or PMA. Tussilagone inhibited PMA-induced activation (phosphorylation) of inhibitory kappa B kinase (IKK), and thus phosphorylation and degradation of inhibitory kappa Ba (IκBα). Tussilagone inhibited PMA-induced phosphorylation and nuclear translocation of nuclear factor kappa B (NF-κB) p65. This, in turn, led to the down-regulation of MUC5AC protein production in NCI-H292 cells. These results suggest that tussilagone can regulate the production and gene expression of mucin by acting on airway epithelial cells through regulation of NF-κB signaling pathway.

Keyword

Airway; Epithelium; Gene expression; Mucin; Tussilagone

MeSH Terms

Blotting, Western
Down-Regulation
Enzyme-Linked Immunosorbent Assay
Epidermal Growth Factor
Epithelial Cells*
Epithelium
Gene Expression*
Mucins*
NF-kappa B
Phosphorylation
Phosphotransferases
Tussilago
Epidermal Growth Factor
Mucins
NF-kappa B
Phosphotransferases
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