Korean J Radiol.  2018 Oct;19(5):950-956. 10.3348/kjr.2018.19.5.950.

Effect of Poly(Lactide-Co-Glycolide) Nanoparticles on Local Retention of Fluorescent Material: An Experimental Study in Mice

Affiliations
  • 1Department of Radiology, Seoul Metropolitan Government-Seoul National University Boramae Hospital, Seoul 07061, Korea.
  • 2Department of Radiology, Seoul National University Bundang Hospital, Seongnam 13620, Korea. eugene801027@gmail.com
  • 3College of Pharmacy, Chung-Ang University, Seoul 06911, Korea.
  • 4College of Pharmacy, Dankook University, Cheonan 31116, Korea.

Abstract


OBJECTIVE
Poly(lactide-co-glycolide) (PLGA) nanoparticles are promising materials for the development of new drug-releasing systems. The purpose of this study was to evaluate the in vivo retention time of materials loaded in nanoparticles as compared with that of the material alone by in vivo imaging in nude mice.
MATERIALS AND METHODS
Mice (n = 20) were injected with 0.1 mL fluorescent material 1,1"²-dioctadecyl-3,3,3"²,3"² tetramethylindotricarbocyanine iodide (DiR)-loaded PLGA nanoparticles (200 nm) into the right paraspinal muscle, and the same volume of pure DiR solution was injected into the left paraspinal muscle. Fluorescence images were obtained using an in vivo optical imaging system. Fluorescent images were taken 1 day after the injection, and seven more images were taken at 1-week intervals. Image analysis was done with ImageJ program, and one region of interest was chosen manually, which corresponded to the highest signal-intensity area of fluorescence signal intensity.
RESULTS
After 7 weeks, 12 mice showed a right-sided dominant signal, representing the DiR loaded PLGA nanoparticles; 5 mice showed a left-side dominant signal, representing the free DiR solution; and 3 mice showed no signal at all beginning 1 day after the injection. During the 7-week period, the mean signal intensities of the free DiR solution and DiR-loaded PLGA nanoparticles diverged gradually. On day 1, the mean signal intensity of free DiR solution was significantly higher than that of DiR-loaded PLGA (p < 0.001). Finally, by week 7, DiR-loaded PLGA express significantly high signal intensity compared with free DiR solution (p = 0.031).
CONCLUSION
The results of the current study suggested that therapeutic agents bound to PLGA nanoparticles may exhibit prolonged retention times.

Keyword

Nanoparticle; DiR; Local retention time; Animal study; In vivo imaging; Spine intervention

MeSH Terms

Animals
Fluorescence
Mice*
Mice, Nude
Nanoparticles*
Optical Imaging
Paraspinal Muscles
Polyglactin 910*
Polyglactin 910

Figure

  • Fig. 1 Flowchart shows experimental animal number.

  • Fig. 2 Changes in fluorescence signal with time.

  • Fig. 3 In vivo fluorescence images of mouse 6.Left paraspinal muscle, pure DiR solution; right paraspinal muscle, DiR-loaded PLGA nanoparticles. Signal intensity of pure DiR solution (dot lines), injected at left paraspinal muscle, shows bright yellow signal intensity on day 1, maintained until week 3, and slightly decreased from week 4. On other hand, DiR-loaded PLGA nanoparticles (arrows), injected at right paraspinal muscle, shows gradual signal increase. At end of study on week 7, DiR-loaded PLGA nanoparticles of right paraspinal muscle express bright yellow spot, whereas pure DiR solution in contralateral side shows reddish color. DiR = 1,1′-dioctadecyl-3,3,3′,3′ tetramethylindotricarbocyanine iodide, L = left, PLGA = poly(lactide-co-glycolide), R = right

  • Fig. 4 In vivo fluorescence images of mouse 3.Left paraspinal muscle, pure DiR solution; right paraspinal muscle, DiR-loaded PLGA nanoparticles. On day 1, both sides show similar bright yellow signal intensity. Signal intensity of pure DiR solution (dot lines), injected at left paraspinal muscle, shows gradual signal decrease. DiR-loaded PLGA nanoparticles (arrows), injected at right paraspinal muscle, express bright yellow signal during 7 weeks. At end of study, DiR-loaded PLGA nanoparticles of right paraspinal muscle still express bright yellow spot, whereas pure DiR solution in contralateral side shows no measurable signal intensity.


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