Diabetes Metab J.  2018 Feb;42(1):74-81. 10.4093/dmj.2018.42.1.74.

Lack of Evidence of the Role of APOA5 3’UTR Polymorphisms in Iranian Children and Adolescents with Metabolic Syndrome

• Salehi S
• Emadi-Baygi M
• Rezaei M
• Kelishadi
• Nikpour P

• KMID: 2409188
• DOI: http://doi.org/10.4093/dmj.2018.42.1.74

Abstract

BACKGROUND
Metabolic syndrome (MetS) is a complex and multifactorial disorder characterized by insulin resistance, dyslipidaemia, hyperglycemia, abdominal obesity, and elevated blood pressure. The apolipoprotein A5 (APOA5) gene variants have been reported to correlate with two major components of MetS, including low levels of high density lipoprotein cholesterol (HDL-C) and high levels of triglyceride. In the present study, we explored the associations between five single nucleotide polymorphisms (SNPs) of APOA5 gene and the MetS risk.
METHODS
In a case-control design, 120 Iranian children and adolescents with/without MetS were genotyped by polymerase chain reaction-sequencing for these SNPs. Then, we investigated the association of SNPs, individually or in haplotype constructs, with MetS risk.
RESULTS
The rs34089864 variant and H1 haplotype (harboring the two major alleles of rs619054 and rs34089864) were associated with HDL-C levels. However, there was no significant association between different haplotypes/individual SNPs and MetS risk.
CONCLUSION
These results presented no association of APOA5 3'UTR SNPs with MetS. Further studies, including other polymorphisms, are required to investigate the involvement of APOA5 gene in the genetic susceptibility to MetS in the pediatric age group.

Keyword

Apolipoprotein A-V; Haplotypes; Metabolic syndrome; miR-TS-SNP

MeSH Terms

Adolescent*
Alleles
Apolipoproteins
Blood Pressure
Case-Control Studies
Child*
Cholesterol, HDL
Genetic Predisposition to Disease
Haplotypes
Humans
Hyperglycemia
Insulin Resistance
Obesity, Abdominal
Polymorphism, Single Nucleotide
Triglycerides
Apolipoproteins
Cholesterol, HDL

Figure

• 

  Fig. 1 Haplotype characterization and frequencies of apolipoprotein A5 (APOA5) polymorphisms in pooled metabolic syndrome and control groups constructed by Haploview software.

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