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Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry in Clinical Microbiology: What Are the Current Issues?

van Belkum A, Welker M, Pincus D, Charrier J, Girard V

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized the identification of microbial species in clinical microbiology laboratories. MALDI-TOF-MS has swiftly become the new gold-standard method owing to...
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Phenotypic and Genomic Characterization of AmpC-Producing Klebsiella pneumoniae From Korea

Palmieri M, Schicklin , Pelegrin A, Chatellier S, Franceschi C, Mirande C, Park YJ, van Belkum A

The prevalence of multidrug-resistant gram-negative bacteria has continuously increased over the past few years; bacterial strains producing AmpC β-lactamases and/or extended-spectrum β-lactamases (ESBLs) are of particular concern. We combined high-resolution...
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Rapid Clinical Bacteriology and Its Future Impact

van Belkum A, Durand G, Peyret M, Chatellier S, Zambardi G, Schrenzel , Shortridge D, Engelhardt A, Dunne WM

Clinical microbiology has always been a slowly evolving and conservative science. The sub-field of bacteriology has been and still is dominated for over a century by culture-based technologies. The integration...
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Characterization of Plasmid-Mediated AmpC and Carbapenemases among Iranain Nosocomial Isolates of Klebsiella pneumoniae Using Phenotyping and Genotyping Methods

Japoni-Nejad A, Ghaznavi-Rad E, van Belkum A

Objectives Plasmid-mediated AmpC β-lactamases (PMABLs) and carbapenemases are emerging groups of antimicrobial-resistance determinants. The aims of the study were to evaluate the occurrence of PMABLs and carbapenemases in clinical isolates of...
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