Tissue Eng Regen Med.  2018 Feb;15(1):49-61. 10.1007/s13770-017-0105-7.

Adipose Stem Cells with Conditioned Media for Treatment of Acne Vulgaris Scar

Affiliations
  • 1Department of Plastic and Reconstructive Surgery, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea. rhie@catholic.ac.kr
  • 2Department of Molecular Biomedicine, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea.

Abstract

This study was to investigate the effect of subcutaneous injection of the adipose stem cells (ASCs) with conditioned media (CM) in the treatment of acne vulgaris scar. We used Adult male New Zealand white rabbit ears as an animal model and induced acne formation by Kignman method. Adipose tissue was isolated and harvested from the scapula of rabbits, and ASCs were cultured and expanded until passage 1. There have four groups in our experiment, include phosphate buffered saline (PBS), ASCs with PBS (ASC + PBS), CM, and ASCs with CM (ASC + CM) group. This solution of 0.6 ml injected to subcutaneous in each group. ASC + PBS and ASC + CM groups were containing ASCs of 5.0 × 106 cells/ml. We analyzed the treatment of 4 groups to scar tissue after 2 and 4 weeks by hematoxylin and eosin stain, immunohistochemistry, and RNA expression level of tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α), and matrix metalloproteinase-2 (MMP-2). Also, the expression of keratin 16 (K16) was detected by western blot analysis. H&E stain showed that infiltration of inflammation cells was significantly reduced at 2 and 4 weeks, as well as re-epithelialization was improved in the ASC + CM group. The ASC + CM gourp was reduced both expression levels of TNF-α, IL-1α, and MMP-2 and K16 protein level. In conclusion, the ASCs with CM has a significant curative effect on acne vulgaris scar, more to the point, the CM has a key role on treatment. It could be applied to a therapeutic approach to regenerate to treat acne vulgaris scar.

Keyword

Acne vulgaris scar; Adipose stem cell; Acne scar model; Conditioned media; Keratin 16

MeSH Terms

Acne Vulgaris*
Adipose Tissue
Adult
Blotting, Western
Cicatrix*
Culture Media, Conditioned*
Ear
Eosine Yellowish-(YS)
Hematoxylin
Humans
Immunohistochemistry
Inflammation
Injections, Subcutaneous
Keratin-16
Male
Matrix Metalloproteinase 2
Methods
Models, Animal
Necrosis
New Zealand
Rabbits
Re-Epithelialization
RNA
Scapula
Stem Cells*
Culture Media, Conditioned
Eosine Yellowish-(YS)
Hematoxylin
Keratin-16
Matrix Metalloproteinase 2
RNA
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