J Korean Ophthalmol Soc.  2018 Feb;59(2):130-136. 10.3341/jkos.2018.59.2.130.

Effect of Particulate Matter on the NLRP3 Inflammasomes in Ocular Tissues and Cervical Lymph Nodes

• Kang SY
• Kang B
• Kim HM
• Song JS

• KMID: 2403805
• DOI: http://doi.org/10.3341/jkos.2018.59.2.130

Abstract

PURPOSE
To evaluate changes of the NLRP3 inflammasome complex and proinflammatory cytokine interleukin (IL)-1β in ocular tissues and cervical lymph nodes when the ocular surface was exposed to particulate matter.
METHODS
Five-week-old Sprague Dawley male rats, each weighing 250-300 g, were used in this study. The rats were randomly divided into two groups involving the TiOâ‚‚-exposed group (n = 12) and control group (n = 12). Rats in the TiOâ‚‚-exposed group were exposed to airborne TiOâ‚‚ microparticles in the exposure chamber twice daily for 2 hours for 5 days. The mean particulate matter (PM) 10 concentration in the exposure chamber was 331 ± 83 µg/m³. The score of corneal fluorescein staining for evaluating ocular surface damage and the size of cervical lymph nodes for evaluating the immune response in the lymphatic drainage pathway were measured. The expression of the nucleotide-binding domain leucine-rich repeats, pyrin domain-containing 3 (NLRP3) inflammasome complex (NLRP3, apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (CARD) domain [ASC], and caspase-1) and IL-1β was quantitatively evaluated.
RESULTS
In the TiO₂-exposed group, the mean corneal stain score was significantly increased compared to that of the control group (p = 0.002), and the size of the cervical lymph nodes in the TiO₂-exposed group was greater than that of the control group (p = 0.004). The expression of NLRP3 and ASC was significantly increased in the corneo-conjunctival tissue and cervical lymph nodes after TiO₂ exposure (both, p = 0.037). Inactive precursor forms of caspase-1 and IL-1β were decreased, but active forms of caspase-1 and IL-1β were increased after TiO₂ exposure (both, p = 0.037).
CONCLUSIONS
When the ocular surface was exposed to ambient particulate matter, the signal pathway of the NLRP3 inflammasome complex was activated, resulting in increased expression of the proinflammatory cytokine, IL-1β. The results suggested that the NLRP3 inflammasome complex was involved in the pathogenesis of ocular surface inflammation caused by exposure to particulate matter.

Keyword

Dry eye; IL-1β; NLRP3 inflammasome; Particulate matter

MeSH Terms

Animals
Drainage
Fluorescein
Humans
Inflammasomes*
Inflammation
Interleukins
Lymph Nodes*
Male
Particulate Matter*
Rats
Signal Transduction
Fluorescein
Inflammasomes
Interleukins
Particulate Matter

Figure

• 

  Figure 1 The mean corneal staining scores of the control and TiO2-exposed groups were compared. (A) Representative photographs of rat eyes after fluorescein staining in both groups. (B) A comparison of the mean corneal staining scores between the two groups. An asterisk (*) indicates a p-value < 0.05 by the Mann-Whitney U test (p = 0.002). Standard deviations are indicated by error bars.

• 

  Figure 2 The mean relative size of cervical lymph nodes in the control and TiO2-exposed groups were compared. (A) Representative photographs of cervical lymph nodes in both groups. (B) A comparison of cervical lymph node size between the two groups. An asterisk (*) indicates a p-value < 0.05 by the Mann-Whitney U test (n = 6, each group). Standard deviations are indicated by error bars. LN = lymph node.

• 

  Figure 3 The expression of nucleotide-binding domain leucine-rich repeats, pyrin domain-containing 3 (NLRP3) and apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (CARD) domain (ASC) in the corneo-conjunctival tissues in the control and TiO2-exposed groups (n = 3, each group). (A) A representative example of a Western blot gel image and densitometry analysis for NLRP3. (B) A representative example of a Western blot gel image and densitometry analysis for ASC. β-Actin was used as an internal loading control for the Wetern blot. An asterisk (*) indicates a significant difference (p-value < 0.05) in the expression of NLRP3 and ASC in the TiO2-exposed group compared with the control group by the Mann-Whitney U test. Standard deviations are indicated by error bars.

• 

  Figure 4 The expression of inactive precursor and active forms of caspase-1 and interleukin (IL)-1β in the corneo-conjunctival tissues in the control and TiO2-exposed groups (n = 3, each group). (A) A representative example of a Western blot gel image and densitometry analysis for pro-caspase-1 and caspase-1. (B) A representative example of a Western blot gel image and densitometry analysis for pro-IL-1β and IL-1β. β-Actin was used as an internal loading control for the Wetern blot. An asterisk (*) indicates a significant difference (p-value < 0.05) in the expression of inactive precursor and active forms of caspase-1 and IL-1β in the TiO2-exposed group compared with the control group by the Mann-Whitney U test. Standard deviations are indicated by error bars.

• 

  Figure 5 The expression of nucleotide-binding domain leucine-rich repeats, pyrin domain-containing 3 (NLRP3) and apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (CARD) domain (ASC) in the cervical lymph nodes in the control and TiO2-exposed groups (n = 3, each group). (A) A representative example of a Western blot gel image and densitometry analysis for NLRP3. (B) A representative example of a Western blot gel image and densitometry analysis for ASC. β-Actin was used as an internal loading control for the Wetern blot. An asterisk (*) indicates a significant difference (p-value < 0.05) in the expression of NLRP3 and ASC in the TiO2-exposed group compared with the control group by the Mann-Whitney U test. Standard deviations are indicated by error bars.

• 

  Figure 6 The expression of inactive precursor and active forms of caspase-1 and interleukin (IL)-1β in the cervical lymph nodes in the control and TiO2-exposed groups (n = 3, each group). (A) A representative example of a W estern blot gel image and densitometry analysis for pro-caspase-1 and caspase-1. (B) A representative example of a Western blot gel image and densitometry analysis for pro-IL-1β and IL-1β. β-Actin was used as an internal loading control for the Wetern blot. An asterisk (*) indicates a significant difference (p-value < 0.05) in the expression of inactive precursor and active forms of caspase-1 and IL-1β in the TiO2-exposed group compared with the control group by the Mann-Whitney U test. Standard deviations are indicated by error bars.

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