J Vet Sci.  2012 Dec;13(4):429-432.

Development of monoclonal antibodies against the abnormal prion protein isoform (PrPres) associated with chronic wasting disease (CWD)

• Jeong HJ
• Lee NH
• Lee JB
• Park SY
• Song CS
• Seo KH
• Kim DW
• Kim YS
• Choi IS

Abstract

Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrPres) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.

Keyword

CWD; elk; monoclonal antibody; prion; PrPres

MeSH Terms

Animals
Antibodies, Monoclonal
Blotting, Western
Brain
Mice
Mice, Knockout
Stress, Psychological
Wasting Disease, Chronic

Figure

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  Fig. 1 Identification of monoclonal antibodies (mAbs) reactive to elk PrPC and PrPres by Western blot. Con: brain homogenates of uninfected elk, Inf: brain homogenates of CWD-infected elk, PK: proteinase K, -: untreated brain homogenates, +: brain homogenates treated with PK.

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