Ann Lab Med.  2015 Nov;35(6):618-623. 10.3343/alm.2015.35.6.618.

Duffy Blood Group Genotyping in Thai Blood Donors

• Nathalang
• Intharanut K
• Siriphanthong K
• Nathalang
• Kupatawintu P

• KMID: 2363265
• DOI: http://doi.org/10.3343/alm.2015.35.6.618

Abstract

BACKGROUND
Duffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequence-specific primers (PCR-SSP), and the probability of obtaining compatible blood for alloimmunized patients was assessed.
METHODS
Five hundred blood samples from Thai blood donors of the National Blood Centre, Thai Red Cross Society, were included. Only 200 samples were tested with anti-Fy(a) and anti-Fy(b) using the gel technique. All 500 samples and four samples from a Guinea family with the Fy(a-b-) phenotype were genotyped by using PCR-SSP. Additionally, the probability of obtaining antigen-negative red blood cells (RBCs) for alloimmunized patients was calculated according to the estimated FY allele frequencies.
RESULTS
The FY phenotyping and genotyping results were in 100% concordance. The allele frequencies of FY*A and FY*B in 500 central Thais were 0.962 (962/1,000) and 0.038 (38/1,000), respectively. Although the Fy(a-b-) phenotype was not observed in this study, FY*B(ES)/FY*B(ES) was identified by PCR-SSP in the Guinea family and was confirmed by DNA sequencing.
CONCLUSIONS
Our results confirm the high frequency of the FY*A allele in the Thai population, similar to that of Asian populations. At least 500 Thai blood donors are needed to obtain two units of antigen-negative RBCs for the Fy(a-b+) phenotype.

Keyword

Duffy blood group; Genotyping; PCR-SSP; Allele frequencies; Thais

MeSH Terms

Adult
Alleles
Asian Continental Ancestry Group/genetics
Base Sequence
Blood Donors
DNA/chemistry/genetics/metabolism
Duffy Blood-Group System/*genetics/immunology
Female
Gene Frequency
Genotype
Humans
Isoantibodies/blood/immunology
Male
Middle Aged
Phenotype
Polymerase Chain Reaction
Receptors, Cell Surface/genetics/*immunology
Sequence Analysis, DNA
Thailand
Young Adult
DNA
Duffy Blood-Group System
Isoantibodies
Receptors, Cell Surface

Figure

• 

  Fig. 1 Electropherograms of the FY gene at the GATA box motif of the promoter region, single nucleotide polymorphism (SNP) c.-33T>C (column A) and the FY*A and FY*B polymorphism region, SNP c.125G>A (column B). DNA sequences of the FY*A homozygote (1), the FY*B homozygote (2), the FY*A/FY*B heterozygote (3), and the FY*BES homozygote (4) are amplified in both regions.

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