Yonsei Med J.  2015 Jan;56(1):196-203. 10.3349/ymj.2015.56.1.196.

Attenuation of Peripheral Regulatory T-Cell Suppression of Skin-Homing CD8+T Cells in Atopic Dermatitis

Affiliations
  • 1Department of Dermatology, Yidu Central Hospital, Weifang Medical University, Weifang, P.R. China.
  • 2Department of Health Statistics, Public Health College of Weifang Medical University, Weifang, P.R. China.
  • 3Department of Clinical Laboratory, Yidu Central Hospital, Weifang Medical University, Weifang, P.R. China.
  • 4Department of Dermatology, Weifang Skin Disease Hospital, Weifang, P.R. China.
  • 5Department of Stomatology, Yidu Central Hospital, Weifang Medical University, Weifang, P.R. China. dentistdg@sina.com

Abstract

PURPOSE
Cutaneous lymphocyte-associated antigen (CLA)-expressing CD8+T cells have been known to play an important role in the pathogenesis of atopic dermatitis (AD). However, the mechanisms underlying the loss of self-tolerance remain unclear. Regulatory T cells (Tregs) play a key role in the development of homeostasis in the immune system. We, therefore, hypothesized that a reduced ability of Tregs to inhibit autologous CD8+CLA+T cells might be underlying mechanism in AD.
MATERIALS AND METHODS
CD8+CLA+T cells and Tregs were obtained from the peripheral blood of AD patients and control volunteers. The frequencies of CD8+CLA+T cells were evaluated. The proliferative responses of CD8+CLA+T cells were assessed by flow cytometry, and the levels of transforming growth factor-beta1 (TGF-beta1) and interleukin-10 (IL-10) in culture supernatants were detected by enzyme-linked immunosorbent assay.
RESULTS
Our results revealed higher frequency and increased expression of perforin and granzyme-B in peripheral CD8+CLA+T cells in AD, and lower inhibitory ability of Tregs on proliferation of CD8+CLA+T cells in AD. Meanwhile, the levels of TGF-beta1 produced by Tregs were significantly lower in AD, and anti-TGF-beta1 abolished such suppression.
CONCLUSION
The attenuated inhibitory ability of Tregs on hyper-activated autologous CD8+CLA+T cells, mediated by TGF-beta1, plays an important role in the pathogenesis of AD.

Keyword

Cutaneous lymphocyte-associated antigen; CD8+T cell; regulatory; T cells; atopic dermatitis
Full Text Links
  • YMJ
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2021 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr