J Korean Ophthalmol Soc.  2011 Feb;52(2):233-240.

Effect of Mitomycin C and 5-Fluorouracil on Cultured Human Nasal Mucosa Fibroblasts

• Kim KH
• Kim IT

Abstract

PURPOSE
To investigate the effects of two antimetabolites, mitomycin C (MMC) and 5-fluorouracil (5-FU), on proliferation of cultured human nasal mucosa fibroblasts.
METHODS
Human nasal mucosa fibroblasts were primarily cultured, and exposed to various concentrations of MMC and 5-FU for 5 minutes. Control fibroblasts were exposed to only DMEM media without the drugs. Effect of drugs on cell morphology was observed by phase-contrast microscopy. Cell viability and apoptosis were measured using MTT [3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-tetrazolium bromide] assay and Acridine orange/Hoechst (AO/HO) staining, respectively.
RESULTS
In both experimental groups exposed to MMC and 5-FU, fibroblasts maintained standard spindle shape. The MTT assay showed that both MMC and 5-FU inhibited fibroblast proliferation in a dose dependent manner. AO/HO staining showed apoptotic cells in both experimental groups.
CONCLUSIONS
Both MMC and 5-FU have an antiproliferative effect on fibroblasts in vitro at least through induction of apoptosis. Therefore, adjuvant use of either MMC or 5-FU during endonasal dacryocystorhinostomy may improve the clinical outcome by inhibiting proliferation of the nasal mucosa.

Keyword

5-fluorouracil; Mitomycin C; Nasal mucosa fibroblast

MeSH Terms

Antimetabolites
Apoptosis
Cell Survival
Dacryocystorhinostomy
Fibroblasts
Fluorouracil
Humans
Microscopy, Phase-Contrast
Mitomycin
Nasal Mucosa
Antimetabolites
Fluorouracil
Mitomycin