J Korean Ophthalmol Soc.  2015 Dec;56(12):1939-1944. 10.3341/jkos.2015.56.12.1939.

Effect of Amniotic Membrane Extract on Cultured Human Nasal Mucosa Fibroblasts

Affiliations
  • 1Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. kimkh@cu.ac.kr

Abstract

PURPOSE
To investigate the effects of amniotic membrane extract (AME) on the survival of cultured human nasal mucosa fibroblasts.
METHODS
Primary cultured human nasal mucosa fibroblasts were exposed to 0, 10, 20, or 30 microg/mL AME for 3 days. The survival of the human nasal mucosa fibroblasts was measured using the MTT assay and apoptosis was evaluated with flow cytometry using annexin-V/propidium iodide double staining.
RESULTS
AME decreased significantly in fibroblast proliferation after exposure to 10 microg/mL (p = 0.000), and caused significant apoptosis of the fibroblasts after exposure to 10 microg/mL (p = 0.024).
CONCLUSIONS
AME decreased fibroblast proliferation in vitro at least through induction of apoptosis. Therefore, adjuvant use of AME during endonasal dacryocystorhinostomy may improve clinical outcomes.

Keyword

Amniotic membrane extract; Apoptosis; Nasal mucosa fibroblast
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