J Korean Pediatr Soc.  1997 Aug;40(8):1162-1168.

Acute Renal Failure (ARF) Associated with Yersinia Pseudotuberculosis Infection Diagnosed by Polymerase Chain Reaction

• Park HG
• Ahn BM
• Kim IS
• Kim ER
• Kim JJ
• Kang SO
• Park HH
• Kim KJ

Abstract

The clinical significance of Yersinia pseudotuberculosis (Y. pseudotuberculosis) has recently recognized in various part of the world because it can cause a wide range of clinical problems such as mesenteric lymphadenitis, septicemia, reactive arthritis, terminal ileitis, erythema nodosum and acute renal failure. we experienced a case of acute renal failure associated with Y. pseudotuberculosis infection. We applied a nested polymerase chain reaction method for rapid diagnosis of Y. pseudotuberculosis infection. DNA was extracted from standard strains of Y. pseudotuberculosis, Y. enterocolitica, serial blood samples, urine, and mountain water by phenol-chloroform method. Using specific primers, we amplified inv and ail gene from extracted DNA samples by PCR method. The patient' serotype was Y. pseudotuberculosis 2a and it's titer was 1:40 initially, after 2 weeks the titer increased to 1:160. Stool culture was negative. Inv gene amplification was positive in patient's urine, febrile stage blood, and mountain water. The nested polymerase chain reaction method can be used clinically for rapid diagnosis of Y. pseudotuberculosis infection. So we report here the clinical findings and PCR method of this case with brief review of the literatures.

Keyword

Yersinia pseudotuberculosis; Acute renal failure; Polymerase chain reaction

MeSH Terms

Acute Kidney Injury*
Arthritis, Reactive
Crohn Disease
Diagnosis
DNA
Erythema Nodosum
Gene Amplification
Mesenteric Lymphadenitis
Polymerase Chain Reaction*
Sepsis
Water
Yersinia pseudotuberculosis*
Yersinia*
DNA
Water