Nutr Res Pract.  2013 Jun;7(3):178-184.

Interaction of physical trainings and coffee intakes in fuel utilization during exercise in rats

Affiliations
  • 1Department of Food and Nutrition, Duksung Women's University, 419 Ssangmun-dong, Dobong-gu, Seoul 132-714, Korea. yunokcho@duksung.ac.kr

Abstract

This study investigates the impact of exercises, coffee intakes, and physical trainings on fuel utilization in rats. Ninety-six rats were fed a control diet with either water (C) or coffee (CF; 0.12 g freeze-dried instant coffee/100 g body weight/d). Additionally, the animals go through physical training (TC and TCF) or no training (NTC and NTCF) for 4 weeks. For physical training, animals have to exercise on treadmills for 30 minutes (5 d per week, 15degrees incline, 0.5-0.8 km/h). At the end of week 4, the animals in each group were subdivided into three exercise groups: before exercise (BE), during exercise (DE), and after exercise (AE). The DE rats exercised on treadmills for 1 hour immediately before being sacrificed. Hemoglobin, hematocrit, glucose, glycogen, protein, triglyceride (TG), and free fatty acid (FFA) levels in the plasma, liver, and skeletal muscle of the rats were compared accordingly. Organ weights were also measured. Coffee-training interaction had a significant impact on heart weight, visceral fat, hemoglobin, hematocrit, liver glycogen in DE and AE, and liver triglyceride in DE and AE. Exercise (meaning exercised on a treadmill for 1 hour immediately before being sacrificed) training interaction was significant in liver glycogen, muscle glycogen in control diet and control diet with coffee, FFA and muscle TG levels at control diet with coffee group. Exercise-coffee interactions significantly influenced the FFA with no training groups. Exercise-coffee-training interaction significantly effects on FFA, Liver TG and Muscle TG. Coffee intakes can increase lipolysis during exercising but coffee consumptions delay the recovery of liver glycogen levels in trained rats after exercising. Coffee intakes can increase lipolysis during exercising but coffee consumptions delay the recovery of liver glycogen levels in trained rats after exercising. Coffee can be an effective ergogenic aid during exercise for physically trained rats.

Keyword

Coffee; training; exercise; glycogen; free fatty acid

MeSH Terms

Animals
Coffee
Diet
Exercise
Glucose
Glycogen
Heart
Hematocrit
Hemoglobins
Intra-Abdominal Fat
Lipolysis
Liver
Liver Glycogen
Muscle, Skeletal
Muscles
Organ Size
Plasma
Rats
Water
Coffee
Glucose
Glycogen
Hemoglobins
Liver Glycogen
Water

Reference

1. Green HJ. How important is endogenous muscle glycogen to fatigue in prolonged exercise? Can J Physiol Pharmacol. 1991; 69:290–297.
Article
2. Hawley JA, Brouns F, Jeukendrup A. Strategies to enhance fat utilisation during exercise. Sports Med. 1998; 25:241–257.
Article
3. Campbell I. Starvation, exercise, injury and obesity. Anaesth Intensive Care Med. 2004; 5:243–248.
Article
4. Choi EY, Cho YO. Moderate physical training can increase muscle glycogen levels but does not alter protein levels with exercise in rats. Nutr Sci. 2006; 9:112–116.
5. Barnett C, Carey M, Proietto J, Cerin E, Febbraio MA, Jenkins D. Muscle metabolism during sprint exercise in man: influence of sprint training. J Sci Med Sport. 2004; 7:314–322.
Article
6. Stuewe SR, Gwirtz PA, Agarwal N, Mallet RT. Exercise training enhances glycolytic and oxidative enzymes in canine ventricular myocardium. J Mol Cell Cardiol. 2000; 32:903–913.
Article
7. Murase T, Haramizu S, Shimotoyodome A, Tokimitsu I, Hase T. Green tea extract improves running endurance in mice by stimulating lipid utilization during exercise. Am J Physiol Regul Integr Comp Physiol. 2006; 290:R1550–R1556.
Article
8. Saldanha Aoki M, Rodriguez Amaral Almeida AL, Navarro F, Bicudo Pereira Costa-Rosa LF, Pereira Bacurau RF. Carnitine supplementation fails to maximize fat mass loss induced by endurance training in rats. Ann Nutr Metab. 2004; 48:90–94.
Article
9. Rogers NL, Dinges DF. Caffeine: implications for alertness in athletes. Clin Sports Med. 2005; 24:e1–e13. x–xi.
Article
10. Spiller MA. The chemical components of coffee. In : Spiller GA, editor. Caffeine. Boca Raton, FL: CRC Press;1998.
11. Costill DL, Dalsky GP, Fink WJ. Effects of caffeine ingestion on metabolism and exercise performance. Med Sci Sports. 1978; 10:155–158.
12. Pasman WJ, van Baak MA, Jeukendrup AE, de Haan A. The effect of different dosages of caffeine on endurance performance time. Int J Sports Med. 1995; 16:225–230.
Article
13. Rodriguez de Sotillo DV, Hadley M, Sotillo JE. Insulin receptor exon 11+/- is expressed in Zucker (fa/fa) rats, and chlorogenic acid modifies their plasma insulin and liver protein and DNA. J Nutr Biochem. 2006; 17:63–71.
Article
14. Venables MC, Hulston CJ, Cox HR, Jeukendrup AE. Green tea extract ingestion, fat oxidation, and glucose tolerance in healthy humans. Am J Clin Nutr. 2008; 87:778–784.
Article
15. Arion WJ, Canfield WK, Ramos FC, Schindler PW, Burger HJ, Hemmerle H, Schubert G, Below P, Herling AW. Chlorogenic acid and hydroxynitrobenzaldehyde: new inhibitors of hepatic glucose 6-phosphatase. Arch Biochem Biophys. 1997; 339:315–322.
Article
16. Johnston KL, Clifford MN, Morgan LM. Coffee acutely modifies gastrointestinal hormone secretion and glucose tolerance in humans: glycemic effects of chlorogenic acid and caffeine. Am J Clin Nutr. 2003; 78:728–733.
Article
17. Shimizu M, Kobayashi Y, Suzuki M, Satsu H, Miyamoto Y. Regulation of intestinal glucose transport by tea catechins. Biofactors. 2000; 13:61–65.
Article
18. Tunnicliffe JM, Erdman KA, Reimer RA, Lun V, Shearer J. Consumption of dietary caffeine and coffee in physically active populations: physiological interactions. Appl Physiol Nutr Metab. 2008; 33:1301–1310.
Article
19. Desbrow B, Hughes R, Leveritt M, Scheelings P. An examination of consumer exposure to caffeine from retail coffee outlets. Food Chem Toxicol. 2007; 45:1588–1592.
Article
20. Lopez-Garcia E, Rodriguez-Artalejo F, Rexrode KM, Logroscino G, Hu FB, van Dam RM. Coffee consumption and risk of stroke in women. Circulation. 2009; 119:1116–1123.
Article
21. Hassid WZ, Abraham S. Chemical procedures for analysis of polysaccharides. Methods Enzymol. 1957; 3:34–50.
22. Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949; 177:751–766.
Article
23. Giegel JL, Ham AB, Clema W. Manual and semi-automated procedures for measurements of triglycerides in serum. Clin Chem. 1975; 21:1575–1581.
Article
24. Raabo E, Terkildsen TC. On the enzymatic determination of blood glucose. Scand J Clin Lab Invest. 1960; 12:402–407.
Article
25. Rogiers V. Stability of the long chain non-esterified fatty acid pattern in plasma and blood during different storage conditions. Clin Chim Acta. 1978; 84:49–54.
Article
26. Hargreaves M. Muscle glycogen and metabolic regulation. Proc Nutr Soc. 2004; 63:217–220.
Article
27. Akerstrom TC, Birk JB, Klein DK, Erikstrup C, Plomgaard P, Pedersen BK, Wojtaszewski J. Oral glucose ingestion attenuates exercise-induced activation of 5'-AMP-activated protein kinase in human skeletal muscle. Biochem Biophys Res Commun. 2006; 342:949–955.
Article
28. Greer F, Hudson R, Ross R, Graham T. Caffeine ingestion decreases glucose disposal during a hyperinsulinemic-euglycemic clamp in sedentary humans. Diabetes. 2001; 50:2349–2354.
Article
29. Keijzers GB, De Galan BE, Tack CJ, Smits P. Caffeine can decrease insulin sensitivity in humans. Diabetes Care. 2002; 25:364–369.
Article
30. Clifford MN. Chlorogenic acids and other cinnamates - nature, occurrence and dietary burden. J Sci Food Agric. 1999; 79:362–372.
Article
31. Herling AW, Burger H, Schubert G, Hemmerle H, Schaefer H, Kramer W. Alterations of carbohydrate and lipid intermediary metabolism during inhibition of glucose-6-phosphatase in rats. Eur J Pharmacol. 1999; 386:75–82.
Article
32. McCarty MF. A chlorogenic acid-induced increase in GLP-1 production may mediate the impact of heavy coffee consumption on diabetes risk. Med Hypotheses. 2005; 64:848–853.
Article
33. Ma YT. Homeostasis and stress in sports and exercise. Biomedical Acupuncture for Sports and Trauma Rehabilitation:Dry Needling Techniques. St. Louis, MO: Churchill Livingstone/Elsevier;2011. p. 6–19.
34. Casal DC, Leon AS. Failure of caffeine to affect substrate utilization during prolonged running. Med Sci Sports Exerc. 1985; 17:174–179.
Article
35. Astrup A, Breum L, Toubro S, Hein P, Quaade F. The effect and safety of an ephedrine/caffeine compound compared to ephedrine, caffeine and placebo in obese subjects on an energy restricted diet. A double blind trial. Int J Obes Relat Metab Disord. 1992; 16:269–277.
36. van den Berghe G. The role of the liver in metabolic homeostasis: implications for inborn errors of metabolism. J Inherit Metab Dis. 1991; 14:407–420.
Article
37. Wende AR, Huss JM, Schaeffer PJ, Giguère V, Kelly DP. PGC-1alpha coactivates PDK4 gene expression via the orphan nuclear receptor ERRalpha: a mechanism for transcriptional control of muscle glucose metabolism. Mol Cell Biol. 2005; 25:10684–10694.
Article
38. Tsuda T, Horio F, Uchida K, Aoki H, Osawa T. Dietary cyanidin 3-O-β-D-glucoside-rich purple corn color prevents obesity and ameliorates hyperglycemia in mice. J Nutr. 2003; 133:2125–2130.
Article
39. Wolfram S, Wang Y, Thielecke F. Anti-obesity effects of green tea: from bedside to bench. Mol Nutr Food Res. 2006; 50:176–187.
Article
40. Choi EY, Jang JY, Cho YO. Coffee intake can promote activity of antioxidant enzymes with increasing MDA level and decreasing HDL-cholesterol in physically trained rats. Nutr Res Pract. 2010; 4:283–289.
Article
41. Lemon PW. Is increased dietary protein necessary or beneficial for individuals with a physically active lifestyle? Nutr Rev. 1996; 54:S169–S175.
Article
42. Andersen LL, Tufekovic G, Zebis MK, Crameri RM, Verlaan G, Kjaer M, Suetta C, Magnusson P, Aagaard P. The effect of resistance training combined with timed ingestion of protein on muscle fiber size and muscle strength. Metabolism. 2005; 54:151–156.
Article
43. Lemmen HJ, Alderliesten RC, Benedictus R. Fatigue initiation behaviour throughout friction stir welded joints in AA2024-T3. Int J Fatigue. 2010; 32:1928–1936.
Article
44. Ma YT. Human brain plasticity, sports, and sports injuries. Biomedical Acupuncture for Sports and Trauma Rehabilitation: Dry Needling Techniques. St. Louis, MO: Churchill Livingstone/Elsevier;2011. p. 20–25.
45. You L, Zhao M, Regenstein JM, Ren J. In vitro antioxidant activity and in vivo anti-fatigue effect of loach (Misgurnus anguillicaudatus) peptides prepared by papain digestion. Food Chem. 2011; 124:188–194.
Article
46. Sedehi D, Ashley EA. Defining the limits of athlete's heart: implications for screening in diverse populations. Circulation. 2010; 121:1066–1068.
47. Irving BA, Davis CK, Brock DW, Weltman JY, Swift D, Barrett EJ, Gaesser GA, Weltman A. Effect of exercise training intensity on abdominal visceral fat and body composition. Med Sci Sports Exerc. 2008; 40:1863–1872.
Article
48. Morck TA, Lynch SR, Cook JD. Inhibition of food iron absorption by coffee. Am J Clin Nutr. 1983; 37:416–420.
Article
49. Peeling P, Dawson B, Goodman C, Landers G, Wiegerinck ET, Swinkels DW, Trinder D. Cumulative effects of consecutive running sessions on hemolysis, inflammation and hepcidin activity. Eur J Appl Physiol. 2009; 106:51–59.
Article
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