Nutr Res Pract.  2010 Aug;4(4):283-289.

Coffee intake can promote activity of antioxidant enzymes with increasing MDA level and decreasing HDL-cholesterol in physically trained rats

Affiliations
  • 1Department of Food & Nutrition, Duksung Women's University, 419 Ssangmun-dong, Dobong-gu, Seoul 132-714, Korea. yunokcho@duksung.ac.kr

Abstract

This study investigated the effect of coffee intake and exercise on the antioxidative activity and plasma cholesterol profile of physically trained rats while they were exercising. Forty eight rats were under either the control diet with water (C) or control diet with coffee (CF) and at the same time they were given physical training for 4 weeks. In terms of physical training, the rats were exercised on a treadmill for 30 minutes everyday. At the end of 4 weeks, animals in each dietary group were subdivided into 3 groups: before-exercise (BE); during-exercise (DE); after-exercise (AE). Animals in the DE group were exercised on a treadmill for one hour, immediately before being sacrificed. Animals in the AE group were allowed to take a rest for one hour after exercise. TG levels were significantly high in coffee intake group than in control group. Also TG level of AE group was significantly higher than that of BE group. Exercise and coffee-exercise interaction effects were significant in total cholesterol (P = 0.0004, 0.0170). The AE of coffee intake group showed highest total cholesterol levels. HDL-cholesterol was significantly lower in coffee intake group than in control group. Coffee, exercise, and coffee-exercise interaction effects were significant in SOD (P = 0.0001, 0.0001, and 0.0001). The AE and BE of coffee intake group showed higher SOD levels than the other four groups. Catalase activities were significantly higher in coffee intake group than control group. No significant main effect was found in GSH/GSSG. Coffee, exercise, and coffee-exercise interaction effects were significant in MDA levels (P = 0.0464, 0.0016, and 0.0353). The DE and AE of coffee intake group and the DE of control group showed higher MDA levels than the BE of control group. Therefore, coffee intake can promote activities of antioxidant enzyme but it also increases MDA and decreases HDL-cholesterol in physically trained rats.

Keyword

Freeze dried instant coffee; antioxidant enzymes; MDA; cholesterol; physical training

MeSH Terms

Animals
Catalase
Cholesterol
Coffee
Diet
Plasma
Rats
Water
Catalase
Cholesterol
Coffee
Water

Figure

  • Fig. 1 The effect of coffee intake and exercise on total cholesterol (Tcol) in physically trained rats. Which are determined by two-way ANOVA. Data were means and letters in the same figure were significantly different at P < 0.05. Control: control diet + physical training, coffee: coffee intake + physical training. BE: before exercise, DE: during exercise, AE: after exercise

  • Fig. 2 The effect of coffee intake and exercise on superoxide dismutase (SOD) in physically trained rats. Which are determined by two-way ANOVA. Data were means, and letters in the same figure were significantly different at P < 0.05. Control: control diet + physical training, coffee: coffee intake + physical training. BE: before exercise, DE: during exercise, AE: after exercise

  • Fig. 3 The effect of coffee intake and exercise on malondialdehide (MDA) in physically trained rats. Which are determined by two-way ANOVA. Data were means, and letters in the same figure were significantly different at P < 0.05. Control: control diet + physical training, coffee: coffee intake + physical training. BE: before exercise, DE: during exercise, AE: after exercise


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