Experimental Animal Model of Bacterial Prostatitis

Lee, Yong Seok; Shin, Myung Sik; Cho, Yong Hyun

Abstract

PURPOSE: Bacterial prostatitis commonly occurs in adult males but the definite diagno sis and the treatment of the disease are not clearly defined yet. The target of this study is to develop a well-defined experimental model of the prostatitis.
MATERIALS AND METHODS
In acute prostatitis model, 30 male Wistar rats weighing 250- 350g were infected with Escherichia coli (O2:K1:H(-)) isolated from the first urine of the acute bacterial prostatitis patients. E. coli was inoculated to the prostate, to the bladder, and to the prostatic urethra. 12 control animals were inoculated with sterilized PBS (phosphate buffered saline, pH 7.2) by the same routes. Bacterial culture and histopathologic examinations were done. The chronic bacterial prostatitis was also produced with 30 male Wistar rats with the most reliable method resulted from the acute experimental model. Bacterial culture and histopathologic examinations were done.
RESULTS
All three routes of inoculation in the acute prostatitis model produced inflam mation of prostate. In the case of bacterial inoculation through the prostatic urethra, the whole part of the prostate was infected without infecting the upper urinary tract. Thus this method was used for the chronic prostatitis model. The inflammation pro gressed with the similar pattern of adult human after three weeks. It successfully developed chronic bacterial prostatitis which was confirmed by the histopathologic study.
CONCLUSIONS
This study showed the injection of E. coli to the prostatic urethra was the most simple and reproducible method to produce the bacterial prostatitis in the animal model without infecting the upper urinary tract.