Korean J Thorac Cardiovasc Surg.  1999 Nov;32(11):998-1003.

Prevention of Calcification in Glutaraldehyde Treated Bovine Pericardium with Combined Treatment of Sodium Dodecyl Sulfate ( SDS ) and Glutamate

• Won TH
• Jang JW
• Yoo JY
• Ahnn JH

Abstract

BACKGROUND: Bovine pericardium treated with glutaraldehyde(GA) is one of the most popular prosthetic materials. However, its late calcific degeneration after implantation results in early failure of the prosthesis. Therefore, we investigated the effects of combined treatment with sodium dodecyl sulfate(SDS) and glutamate on calcific degeneration of GA treated bovine pericardium. MATERIAL AND METHOD: Sixty square-shaped pieces of bovine pericardia were fixed in 0.625% GA solution with 4g/L MgCl2 6H2O as a control group (group 1). Sixty pieces pretreated with 1% SDS (group 2) and sixty pieces posttreated with 8% glutamate (group 3) were also fixed in the same GA solution. Sixty pieces pretreated with 1% SDS and posttrated with 8% glutamate were also fixed in the same GA solution (group 4). After 1 month of fixation, the pieces were implanted into the belly of sixty Sprague-Dawley rat subdermally and were extracted 1 month, 2 months and 3 months after the implantation. With an atomic absorption spectrophotometry, we measured the calcium amount deposited. RESULT: The calcium deposition in 1 month was 2.01+/-0.13 mg/g in group 1, 1.45+/-0.31 mg/g in group 2, 2.49+/-0.15 mg/g in group 3 and 0.75+/-0.27 mg/g in group 4. In 2 months, it was 3.57+/-0.15 mg/g in group 1, 0.98+/-0.30 mg/g in group 2, 3.46+/-0.12 mg/g in group 3, and 1.48+/-0.39 mg/g in group 4, and 5.45+/-0.42 mg/g in group 1, 2.43+/-0.53 mg/g in group 2, 4.20+/-0.55 mg/g in group 3, and 1.02+/-0.27 mg/g in group 4 in 3 months. The calcium depositions in group 2 and 4 were less than those of group 1 and 3 in 1 month 2, months, and 3 months(p<0.01). The calcium depositions in group 1, 2 and 3 increased with time. However, they remained unchanged in group 4, which was statistically significant(p<0.01).
CONCLUSION
Pretreatment with SDS is effective in reducing calcification of GA treated bovine pericardium, and the combined method of pretreatment with SDS and posttreatment with glutamate was more effective than the other methods.

Keyword

Calcinosis; Glutamate; Pericardium

MeSH Terms

Animals
Calcinosis
Calcium
Glutamic Acid*
Glutaral*
Magnesium Chloride
Pericardium*
Prostheses and Implants
Rats
Rats, Sprague-Dawley
Sodium Dodecyl Sulfate*
Sodium*
Spectrophotometry, Atomic
Calcium
Glutamic Acid
Glutaral
Magnesium Chloride
Sodium
Sodium Dodecyl Sulfate