J Asthma Allergy Clin Immunol.  1999 Feb;19(1):50-61.

The effect of respiratory syncytial virus infection on neutrophil adherence to airway epithelial cells

Abstract

BACKGROUND: Respiratory syncytial virus (RSV) is the most common cause of lower respiratory infection in infants and young children, but the pathogenesis of RSV-induced inflammation is not well defined. MATERIAL AND METHOD: In order to examine the potential interactions between virus-infected airway epithelial cells and neutrophils, we studied the ability of neutrophils to adhere to yirus-infected airway epithelial cell monolayers by myeloperoxidase assay. Also we measured the ability of airway epithelial cells to secrete interleukin-8(IL-8) and inter-cellular adhesion molecule-1(ICAM-1) in virus-infected airway epithelial cell cultures by enzyme-linked immunosorbent assay(ELISA). The degree of IL-8 and ICAM-1 gene expression in the RSV-infected BEAS-2B cell cultures were analyzed by reverse transcription-polymerase chain reaction(RT-PCR).
RESULTS
The RSV-infected BEAS-2B cell resulted in significantly enhanced level of neutrophil adherence compared to the uninfected control(p (0.001). IL-8 and ICAM-1 production significantly increased by RSV infection(p<0.05). There was a significant positive correlation between neutrophil adherence and IL-8 level(r=0.73, p=0.002), and ICAM-1 level (r=0.843, p=0.001) in RSV-infected cells. The degree of both IL-8 and ICAM-1 mRNA expression increased in the RSV-infected cells compared with the uninfected ones.
CONCLUSION
These results suggest that RSV infection significantly enhances the production of IL-8 and ICAM-1 in airway epithelial cells which then results in increased neutrophil adherence.

Keyword

BEAS-2B cell line; IL-8; ICAM-1; respiratory syncytial virus; neutrophil adherence

MeSH Terms

Cell Culture Techniques
Child
Epithelial Cells*
Gene Expression
Humans
Infant
Inflammation
Intercellular Adhesion Molecule-1
Interleukin-8
Neutrophils*
Peroxidase
Respiratory Syncytial Viruses*
RNA, Messenger
Intercellular Adhesion Molecule-1
Interleukin-8
Peroxidase
RNA, Messenger
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