Korean J Physiol Pharmacol.  2006 Jun;10(3):161-165.

Nitric Oxide Donor, NOR-3, Increased Expression of Cyclooxygenase-2, but not of Cyclooxygenase-1 in Cultured VSMC

Affiliations
  • 1Department of Pharmacology, College of Medicine, Yeungnam University, Daegu, Korea. hcchoi@med.yu.ac.kr
  • 2Department of Thoracic & Cardiovascular Surgery, College of Medicine, Yeungnam University, Daegu, Korea.

Abstract

NO and cyclooxygenase-2 (COX-2) are contributes to vascular inflammation induced by various stimulation. The mechanism, which explains a linkage between NO and COX-2, could be of importance in promoting pathophysiological conditions of vessel. We investigated the effects of NO donors on the COX-1 and COX-2 mRNA/protein expression, as well as the nitrite production in culture medium of vascular smooth muscle cell (VSMC). VSMC was primarily cultured from thoracic aorta of rat. In this experiments, COX-1 and COX-2 mRNA/protein expressions were analysed and nitrite productions were investigated using Griess reagent. VSMC did not express COX-2 protein in basal condition (Non-lipopolysaccharide (LPS) stimulated). In LPS-stimulated experiments, after 3 hours of NO donor pretreatment, LPS 10 microgram/ml was treated for 24 hours. COX-1 protein expressions were unchanged by SNP and NOR-3. NOR-3 significantly increased COX-2 mRNA/protein expression under LPS stimulation. In contrast, SNP did not increase COX-2 mRNA/protein expression under LPS stimulation. Nitrite production was higher in NOR-3 treatment than SNP treatment under LPS stimulation. These results suggest that the expression of COX-2 in VSMC is regulated by NOR-3, COX-2 expressions were depending on the types of NO donor and LPS stimulation in VSMC.

Keyword

COX-2; SNAP; SNP; NOR-3; VSMC

MeSH Terms

Animals
Aorta, Thoracic
Cyclooxygenase 1*
Cyclooxygenase 2*
Humans
Inflammation
Muscle, Smooth, Vascular
Nitric Oxide*
Rats
Tissue Donors*
Cyclooxygenase 1
Cyclooxygenase 2
Nitric Oxide
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