Korean J Physiol Pharmacol.  1998 Aug;2(4):511-519.

Protective mechanism of nitric oxide and mucus against ischemia/reperfusion-induced gastric mucosal injury

• Kim H
• Nam K
• Kim KH

Abstract

This study investigated the role of nitric oxide on the oxidative damage in gastric mucosa of rats which received ischemia/reperfusion and its relation to mucus. Nitric oxide synthesis modulators such as L-arginine and NG-nitro-L-arginine methyl ester, and sodium nitroprusside, a nitric oxide donor, were injected intraperitoneally to the rats 30 min prior to ischemia/reperfusion which was induced by clamping the celiac artery and the superior mesenteric artery for 30 min and reperfusion for 1 h. Lipid peroxide production, the contents of glutathione and mucus, and glutathione peroxidase activities of gastric mucosa were determined. Histological observation of gastric mucosa was performed by using hematoxylin-eosin staining and scanning electron microscopy. The result showed that ischemia/reperfusion increased lipid peroxide production and decreased the contents of glutathione and mucus as well as glutathione peroxidase activities of gastric mucosa. Ischemia/reperfusion induced gastric erosion and gross epithelial disruption of gastric mucosa. Pretreatment of L-arginine, a substrate for nitric oxide synthase, and sodium nitroprusside prevented ischemia/reperfusion-induced alterations of gastric mucosa. However, NG-nitro-L-arginine methyl ester, a nitric oxide synthase inhibitor, deteriorated oxidative damage induced by ischemia/reperfusion. In
conclusion
, nitric oxide has an antioxidant defensive role on gastric mucosa by maintaining mucus, glutathione, and glutathione peroxidase of gastric mucosa.

Keyword

Nitric oxide; Mucus; Gastric mucosa; Ischemia/reperfusion

MeSH Terms

Animals
Arginine
Celiac Artery
Constriction
Gastric Mucosa
Glutathione
Glutathione Peroxidase
Humans
Mesenteric Artery, Superior
Microscopy, Electron, Scanning
Mucus*
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase
Nitric Oxide*
Nitroprusside
Rats
Reperfusion
Tissue Donors
Arginine
Glutathione
Glutathione Peroxidase
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Nitroprusside