Korean J Physiol Pharmacol.  1997 Dec;1(6):647-655.

Effects of cyclic nucleotides and glipizide on the cardiovascular response of baclofen in the rats

• Shin IC
• Koh HC
• Ha JH

Abstract

The purpose of present study is to investigate the influence of a spinal gamma-aminobutyric acid B(GABA|B) receptor on a central regulation of blood pressure (BP) and heart rate (HR), and to define its mechanism in the spinal cord. In urethane-anesthetized, d-tubocurarine-paralyzed and artificially ventilated male Sprague-Dawley rats, intrathecal administration of drugs were carried out using injection cannula (33-gauge stainless steel) through the guide cannula (PE 10) which was inserted intrathecally at lower thoracic level through the puncture of a atlantooccipital membrane. Intrathecal injection of an GABA|B receptor agonist, baclofen (30, 60, 100 nmol) decreased both BP and HR dose-dependently. Pretreatment with 8-bromo-cAMP (50 nmol), a cAMP analog, or glipizide (50 nmol), a ATP-sensitive K+ channel blocker, attenuated the depressor and bradycardic effects of baclofen (100 nmol), but not with 8-bromo-cGMP (50 nmol), a cGMP analog. These results suggest that the GABA|B receptor in the spinal cord plays an inhibitory role in central cardiovascular regulation and that this depressor and bradycardic actions are mediated by the decrease of cAMP via the inhibition of adenylate cyclase and the opening of K+ channel.

Keyword

Baclofen; Spinal cord; cAMP; cGMP; Glipizide; Blood pressure; Heart rate

MeSH Terms

8-Bromo Cyclic Adenosine Monophosphate
Adenylyl Cyclases
Animals
Baclofen*
Blood Pressure
Catheters
gamma-Aminobutyric Acid
Glipizide*
Heart Rate
Humans
Injections, Spinal
Male
Membranes
Nucleotides, Cyclic*
Punctures
Rats*
Rats, Sprague-Dawley
Spinal Cord
8-Bromo Cyclic Adenosine Monophosphate
Baclofen
Glipizide
Nucleotides, Cyclic
gamma-Aminobutyric Acid