Korean J Otolaryngol-Head Neck Surg.
2000 Nov;43(11):1196-1201.
The Effect of Glucocorticosteroids on Eosinophil Apoptosis Primed by Cultured Nasal Polyp Epithelial Cell Supernatant
- Affiliations
-
- 1Department of Otolaryngology, College of Medicine, Catholic University of Taegu, Taegu, Korea. hsseung@cuth.cataegu.ac.kr
- 2Department of Clinical Pathology, College of Medicine, Catholic University of Taegu, Taegu, Korea.
- 3Department of Otolaryngology, College of Medicine, Kyungpook National University, Taegu, Korea.
Abstract
-
BACKGROUND AND OBJECTIVES: Eosinophil infiltration into inflammatory site is a characteristic histological finding in patients with allergic rhinitis, bronchial asthma, and nasal polyps. The regulation of eosinophil survival and apoptosis may play a major role in tissue eosinophilia, and glucocorticosteroids (GCs) have been used therapeutically for nasal polyps. The purpose of this study is to investigate the effect of commonly used GCs on eosinophil survival and apoptosis primed by nasal polyp epithelial cells.
MATERIALS AND METHODS
Peripheral blood eosinophils were incubated in increasing concentrations (10-10M) to GCs (triamcinolone, dexamethasone, budesonide, and fluticasone propionate) from January, 1990 to December, 1999 prior to the addition of human nasal polyp epithelial cell conditioned media (HECM). Eosinophil viability was measured with a MTS assay and apoptosis was analyzed with the caspase-3 staining.
RESULTS
GCs suppressed the HECM induced prolongation of eosinophil survival with apoptotic change of cells from 2 days after incubation. Fluticasone propionate showed the strongest effects and triamcinolone showed the weakest effects. CONCLUSION: GCs may diminish eosinophilic infiltration into nasal polyp by decreasing eosinophil viability, and abrogate the promoting effect of nasal epithelial cells.
Keyword
MeSH Terms
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effects of Fungi and Eosinophils on Mucin Gene Expression in Rhinovirus-Infected Nasal Epithelial Cells
- Expression of Cytokines in Coculture Model with Epithelial Cells and Fibroblasts of Nasal Polyp
- A Comparison of Histopathologic Findings in the Nasal Polyp, Maxillary Sinus Mucosa, and Inferior Turbinate Mucosa
- Th2 Responses Elicited by Nasal Epithelial Cells Exposed to House Dust Mite Extract
- Inhibition of Fungi-Induced Nasal Polyp Epithelial Cells Activation by Corticosteroids
