Korean J Nutr.  2005 Jun;38(5):352-363.

Antioxidative Effect of Plant Food Mixtures in Rat Fed on High Fat-High Cholesterol Diet

• Kwak CS
• Kim MY
• Lee MS

Abstract

The previous extensive in vitro studies on the antioxidative activities of a number of Korean grains, vegetables, seaweeds and mushrooms, and the various combinations of these food source exhibited a wide range of antioxidative activities, and four food mixtures composed of 5 kinds of foods (5A, 5B, 5C and 5D) were designed from 16 selective foods showing high antioxidant effect, in vitro, to find the good combinations for the meal planning. Mixture 5B or 5C contained very high levels of total flavonoid and polyphenol, and ethanol extract from 5A, 5B or 5C showed very strong inhibitory effects against in vitro Fe2+-induced lipid peroxidation and ethanol extract from 5B or 5C showed remarkable DPPH radical scavenging effect and lipid peroxide-protein conjugation inhibition effect. And in vivo study was also carried out with two mixtures (5B, 5C). Powders (P5B, P5C) or ethanol extracts (E5B, E5C) of these mixtures were supplemented to Sprague-Dawley rats fed on high fat (15%)-high cholesterol (1%) semipurified diet for 5 weeks. The total antioxidant power in serum was significantly higher in P5B, P5C, E5B and E5C groups than in high fat control group, and ascorbate-Fe2+-induced TBARS was significantly lowered by E5B supplementation in rat liver. In liver tissue, Cu, Zn-SOD activity was significantly higher in P5B and E5B groups than in high fat control group, while catalase or GSH-peroxidase (GPx) activity was not changed by any supplementations. In kidney, Cu, Zn- SOD activity was significantly higher in P5B group than in high fat control group, while GPx activity was not changed by any supplementations. Taken together, mixture 5B and 5C showed very strong antioxidative effects both in vitro and in vivo. Therefore, the ingredient Korean foods of 5B and 5C could be recommended to take a lot together for prevention from age-related chronic diseases.

Keyword

food mixture; ethanol extract; total antioxidant power; ascorbate-Fe2+-induced TBARS; lipid peroxideprotein conjugation

MeSH Terms

Agaricales
Animals
Antioxidants
Catalase
Cholesterol*
Chronic Disease
Diet*
Edible Grain
Ethanol
Kidney
Lipid Peroxidation
Liver
Meals
Plants*
Powders
Rats*
Rats, Sprague-Dawley
Thiobarbituric Acid Reactive Substances
Vegetables
Antioxidants
Catalase
Cholesterol
Ethanol
Powders
Thiobarbituric Acid Reactive Substances