Nutr Res Pract.  2010 Feb;4(1):11-15.

Effect of ethanol extracts from red pepper seeds on antioxidative defense system and oxidative stress in rats fed high-fat, high-cholesterol diet

• Song WY
• Ku KH
• Choi JH

Abstract

The purpose of the present study was to investigate the effect of ethanol extracts from red pepper seeds on the antioxidative defense system and oxidative stress in rats fed a high fat . high cholesterol diet. Rats were divided into four experimental groups which were composed of high fat . high cholesterol diet group (HF), high fat . high cholesterol diet with 0.1% ethanol extracts from red pepper seeds supplemented group (HEA), high fat . high cholesterol diet with 0.2% ethanol extracts from red pepper seeds supplemented group (HEB) and high fat.high cholesterol diet with 0.5% ethanol extracts from red pepper seeds supplemented group (HEC). Supplementation of ethanol extracts from red pepper seeds groups (HEA, HEB and HEC) resulted in significantly increased activities of hepatic glutathione peroxidase and catalase. Hepatic superoxide radical contents in microsome and mitochondria were significantly reduced in the groups supplemented with red pepper seeds ethanol extracts. Hepatic hydrogen peroxide content in the mitochondria was reduced in ethanol extracts from red pepper seeds supplemented groups. TBARS values in the liver were reduced in red pepper seeds ethanol extracts supplemented groups. Especially, HEB and HEC groups were significantly decreased compared to the HF group. Hepatic carbonyl values were significantly reduced in mitochondria in these supplemented groups. These results suggest that red pepper seeds ethanol extracts may reduce oxidative damage, by activation of antioxidative defense system in rats fed high fat . high cholesterol diets.

Keyword

Cholesterol; red pepper seed extracts; free radical; oxidative stress; antioxidative defense system

MeSH Terms

Animals
Capsicum
Catalase
Cholesterol
Diet
Ethanol
Glutathione Peroxidase
Hydrogen Peroxide
Liver
Microsomes
Mitochondria
Oxidative Stress
Rats
Seeds
Superoxides
Thiobarbituric Acid Reactive Substances
Catalase
Cholesterol
Ethanol
Glutathione Peroxidase
Hydrogen Peroxide
Superoxides
Thiobarbituric Acid Reactive Substances

Figure

• 

  Fig. 1 Effects of ethanol extracts from red pepper seeds on hepatic superoxide radical contents in rats fed high fat · high cholesterol diets. Values are the means ± SE (n=10). Those with different superscript letters are significantly different at P < 0.05 by Tukey's test. The experimental groups are the same as in Table 1.

• 

  Fig. 2 Effects of ethanol extracts from red pepper seeds on mitochondrial hydrogen peroxide (H2O2) contents in rats fed high fat · high cholesterol diets. Values are the means ± SE (n=10). Those with different superscript letters are significantly different at P < 0.05 by Tukey's test. The experimental groups are the same as in Table 1.

• 

  Fig. 3 Effects of ethanol extracts from red pepper seeds on hepatic thiobarbituric acid reactive substances (TBARS) values in rats fed high fat · high cholesterol diets. Values are the means ± SE (n=10). Those with different superscript letters are significantly different at P < 0.05 by Tukey's test. The experimental groups are the same as in Table 1.

• 

  Fig. 4 Effects of ethanol extracts from red pepper seeds on hepatic carbonyl value in rats fed high fat · high cholesterol diets. Values are the means ± SE (n=10). Those with different superscript letters are significantly different at P < 0.05 by Tukey's test. The experimental groups are the same as in Table 1. NS : not significant

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