Korean J Nutr.  2011 Dec;44(6):474-480. 10.4163/kjn.2011.44.6.474.

Bioavailability of Aspartic Acid Chelated Calcium in Calcium Deficient Rats

• Park MG
• Ha TY
• Shin KS

• KMID: 2268607
• DOI: http://doi.org/10.4163/kjn.2011.44.6.474

Abstract

Calcium (Ca) is an essential element to maintain body homeostasis. However, many factors disturb calcium absorption. Aspartic acid chelated calcium (AAC) was synthesized by new methods using calcium carbonate and aspartic acid. This study was carried out to investigate the bioavailability of AAC in Ca-deficient rats. The experimental groups were as follows: NC; normal diet control group, CD-C; untreated control group of Ca-deficient (CD) rats, CD-CaCO3; CaCO3 treated group of CD rats, CD-AAC; AAC treated group of CD rats, and CD-SWC; and seaweed-derived Ca treated group of CD rats. The Ca content of various types of Ca was held constant at 32 mg/day, and the four CD groups were fed for 7 days after randomized grouping. Ca content in serum, urine, and feces within feeding periods were analyzed to confirm Ca absorption. Serum Ca content was significantly higher in the CD-AAC (11.24 mg/dL) and CD-SWC (10.12 mg/dL) groups than that in the CD-C (8.6 mg/dL) group 2 hours following the first administration. The Ca content in feces was significantly lower in the CD-AAC (35.4 mg/3 days) and CD-SWC (71.1 mg/3 day) groups than that in the CD-CaCO3 (98.7 mg/3 days) group (p > 0.05). AAC had a 2.3-fold higher absorption rate of Ca than that of SWC. No differences in fibula length were observed in the NC and CD groups. The fibula weights of the CD-AAC (0.33 g) and CD-SWC (0.33 g) groups increased compared to those in the CD-C (0.27 g) group; however, no significant difference was observed between the CD groups. We conclude that bioavailability of AAC is higher than that of seaweed-derived Ca or inorganic Ca. Thus, these findings suggest the AAC has potential as a functional food material related to Ca metabolism.

Keyword

aspartic acid chelated calcium; bioavailability; adsorption; calcium deficient rat

MeSH Terms

Absorption
Adsorption
Animals
Aspartic Acid
Biological Availability
Calcium
Calcium Carbonate
Diet
Feces
Fibula
Functional Food
Homeostasis
Rats
Weights and Measures
Aspartic Acid
Calcium
Calcium Carbonate

Figure

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  Fig. 1 Acute metabolic effects by various forms of calcium supplement. NC: normal control, CD-C: Vehicle administration on calcium deficient rat, CD-CaCO3: CaCO3 administration on calcium deficient rat, CD-AAC: amino acid chelated calcium (AAC) administration on calcium deficient rat, CD-SWC: seaweed-derived calcium administration on calcium deficient rat. Bloods were collected in two hours following first administration. Values are Mean ± SD for 6 rats. Means with different superscript letters are significantly different at p < 0.05 by Duncan's multiple range tests.

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  Fig. 2 Changes of length (A) and weight (B) on fibula by various forms of calcium supplement. NC: normal control, CD-C: Vehicle administration on calcium deficient rat, CD-CaCO3: CaCO3 administration on calcium deficient rat, CD-AAC: amino acid chelated calcium (AAC) administration on calcium deficient rat, CD-SWC: seaweed-derived calcium administration on calcium deficient rat. Fibulas were collected in 7 days following first administration. Values are Mean ± SD for 6 rats. Means with different superscript letters are significantly different at p < 0.05 by Duncan's multiple range tests. ns: not significant.

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