Korean J Nucl Med.  2000 Aug;34(4):344-352.

Effect of Carrier on Labeling and Biodistribution of Re-188-Hydroxyethylidene diphosphonate

• Chang YS
• Jeong JM
• Kim BK
• Cho JH
• Lee DS
• Chung JK
• Lee SJ
• Kim IK
• Lee SE
• Lee MC

Abstract

PURPOSE: Re-188-Hydroxyethylidene diphosphonate (HEDP) is a new cost-effective agent for systemic radioisotope therapy of metastatic bone pain. We investigated the influence of carrier for labeling and biodistribution of Re-188-HEDP using HEDP kit with or without carrier (KReO4). MATERALS AND METHODS: The kits (HEDP 15 mg, gentisic acid 4 mg and SnCl2.2H2O 4.5 mg) with or without carrier (KReO4 0.1 mg) were labeled with Re-188 solution, made available from an in-house generator by boiling for 15 min. We compared the labeling efficiency and stability of carrier-added and carrier-free preparations of Re-188-HEDP. Biodistribution and imaging studies of each preparation were performed in ICR mice (1.85~3.7 MBq/0.1 ml) and SD rats (74.1~85.2 MBq/0.5 ml).
RESULTS
The carrier-added preparation showed high labeling efficiency (95% at pH 5) and high stability in serum (88%, 3 hr). However, the carrier-free preparation showed low labeling efficiency (59% at pH 5) and low stability (43%, 3 hr). The carrier-added preparation showed high uptake in bone and low uptake in stomach and kidneys. However, the carrier-free preparation showed lower uptake in bone and higher uptake in both stomach and kidneys, which is supposed to be due to released perrhenate. The carrier-added preparation also showed better images with higher skeletal accumulation, lower uptake in other organs and lower soft tissue uptake than the carrier-free preparation.
CONCLUSION
The results of these studies clearly demonstrate that addition of carrier perrhenate is required for high labeling efficiency, stability, bone uptake and good image quality of Re-188-HEDP.

Keyword

Re-188-HEDP; Carrier; Labeling efficiency; Stability

MeSH Terms

Animals
Etidronic Acid
Hydrogen-Ion Concentration
Kidney
Mice
Mice, Inbred ICR
Rats
Stomach
Etidronic Acid