Korean J Nephrol.  2002 May;21(3):375-381.

Decreased Expression of Aquaporin-2 Water Channels in the Kidney in Rats Treated with Chlorpropamide

• Kim SW
• Lee JU
• Li YS
• Oh YW
• Kim NH
• Choi KC

Abstract

BACKGROUND: Whether blood glucose levels may change the regulation of aquaporin(AQP) water channels in the kidney was investigated.
METHODS
Male Sprague-Dawley rats were treated with chlorpropamide(40 mg/100 g body weight per day, per oral, for 7 days), and their expression of AQP1-3 and type VI adenylyl cyclase proteins was determined in the kidney.
RESULTS
Following the treatment with chlorpropamide, the blood glucose level was significantly decreased compared with that in the control(64+/-8 vs 106+/-7 mg/dL, n=6 each, p < 0.01). Accordingly, the expression of AQP2 proteins was decreased in the cortex, outer medulla, and inner medulla. The AQP2 targeting was not significantly altered, as evidenced by parallel decreases of its expression in the membrane and the cytoplasmic fractions. No significant changes were observed in the expression of either AQP1 or of AQP3. The protein expression of type VI adenylyl cyclase was not significantly altered.
CONCLUSION
These results suggest that hypoglycemia attenuates the expression of AQP2 water channels in the kidney.

Keyword

Aquaporin water channels; Chlorpropamide; Hypoglycemia

MeSH Terms

Adenylyl Cyclases
Animals
Aquaporin 2*
Aquaporins*
Blood Glucose
Body Weight
Chlorpropamide*
Cytoplasm
Humans
Hypoglycemia
Kidney*
Male
Membranes
Rats*
Rats, Sprague-Dawley
Aquaporin 2
Aquaporins
Blood Glucose
Chlorpropamide