Effects of the composition of peritoneal dialysis solution on phagocytic function of peritoneal macrophage in rats

Choi, Seung Ok; Song, Ki Hak; Han, Byoung Geun; Lee, Sang Bong; Kim, Jeong Min; Lee, Eun Young; Kim, Jin Soo

Abstract

BACKGROUND: Continuous ambulatory peritoneal dialysis (CAPD) has been accepted as one of the long-term treatment modalities for end-stage renal disease and peritonitis is the most important complication in CAPD therapy and first-line defense activity was achieved by peritoneal macrophage(PM). Our study has compared the phagocytic activity in rat PM according to various characteristic CAPD solutions(pH, glucose, Ca++, and erythropoietin).
METHODS
Peritoneal cells were isolated from Sprague-Dawley rat with RPMI(4C(degrees), 100 ml). Various characteristic solutions were prepared. The solutions contained 1.5% or 4.25% dextrous, pH 5.4 or pH adjustment to 7.4, low calcium concentration or high calcium concentration and free erythropoietin or 2000U/L erythropoietin. Phagocytic activity of PM was measured by Candida albicans(ATCC 10231) under the inverted microscope.
RESULTS
There was significantly increased phagocytic activity of rat PM in pH 7.4 solution than in pH 5.4 solution(61.8% vs. 72.4% in 1.5% dextrous solution; p< 0.05, 61.6% vs. 69.2% in 4.25% dextrous solution; p< 0.05) and glucose concentrations affected phagocytic activity in pH 7.4 solution. There was a significantly decreased phagocytic activity of rat PM in low calcium concentration than in high calcium concentration(61.8% vs. 59.1% in 1.5% dextrous solution; p> 0.05, 61.6% vs. 45.3% in 4.25% dextrous solution; p< 0.05) and high glucose concentration affected phagocytic activity in low calcium concentration(p< 0.05). There was a significantly decreased phagocytic activity of rat PM in 2000U/L erythropoietin solution than in free erythropoietin solution(61.8% vs. 59.9% in 1.5% dextrous solution; p> 0.05, 61.6% vs. 52.3% in 4.25% dextrous solution; p< 0.05) and high glucose concentration affected to phagocytic activity in 2000U/L erythropoietin solution. CONCLUSIONS: These findings suggest that phagocytic activity of rat PM was increased in pH 7.4 solution and decreased in low calcium concentration and solution which was added erythropoietin, especially in high glucose solution. But glucose concentration did not affect phagocytic activity of rat PM independently.