Korean J Gynecol Oncol Colposc.  1993 Dec;4(4):47-55.

Flow Cytometric Analysis of DNA Content in Cervical Neoplasia Patients Infected with Human Papillomavirus Types 16 and 18

• Park TK
• Kim SN

Abstract

To investigate the role of human pepillomavirus(HPV) infection on DNA content of cervical cancers, thirty-seven cases af ceryical squamous carcinoma were studied with the method of flow cytometry for deoxyribonucleic acid(DMA) content and Southern blot hybridizalion for typing of HPV type 1.6 or type 18 DWA in cornbination with other elinical pnrameters. There were 18 dipleid cases(48.6%) and 19 aneuploid(51.4%). The mean age of the patients with diplaid and aneuploid tumars was 51.6 years and 53.6 years, respectively, No significant age difference was found between diploid and aneuploid groups. Six of 13(46.2%) carcinnma in eiiu and 13 of 24(54.2%) invasive cervical carcinoma were aneuploid, and t,he proportinn of aneuploid tumors was not significantly different between the two groups. Six of 10 tumors(60.0%) in stage I, 5 of 11 tumors(45.5%) in stage II, 2 of 3 tumors(66.7%) in stage III were aneuploid. The frequency was not significantly different according to the clinical stage, Aneuploidy was present in 50.0% of HPV 16 positive lesion, 66.7% of HPV 18 positive lesion, and 41.7% of HPV 16 and 18 negative lesion. There was no statistically difference in the incidenee of aneuploidy when all HPV lesions were compared. Of 25 patients with HFV 16 or l8 positive lesion, aneuploidy was demonstrated in the specimen of four of eight patients with careinoma in situ and nine of 17 with invasive cervical carcinoma. In suromary, we found thst the ineidere of DNA aneuploidy was somewhat higher when HPV 18 positive lesion was compared to HPV 16 and 18 negative lesion but the difference was not statistically significant.

MeSH Terms

Aneuploidy
Blotting, Southern
Carcinoma, Squamous Cell
Diploidy
DNA*
Flow Cytometry
Human papillomavirus 16
Human papillomavirus 18
Humans
Humans*
DNA