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An Enzymolysis-Assisted Agrobacterium tumefaciens-Mediated Transformation Method for the Yeast-Like Cells of Tremella fuciformis

Wang Y, Xu D, Sun X, Zheng L, Chen L, Ma A

Agrobacterium tumefaciens-mediated transformation (ATMT), as a simple and versatile method, achieves successful transformation in the yeast-like cells (YLCs) of Tremella fuciformis with lower efficiency. Establishment of a more efficient transformation...
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First Molecular Diagnosis of a Patient with Unverricht-Lundborg Disease in Korea

Kim KH, Song JS, Park CW, Ki CS, Heo K

Unverricht-Lundborg disease (ULD) is a form of progressive myoclonus epilepsy characterized by stimulation-induced myoclonus and seizures. This disease is an autosomal recessive disorder, and the gene CSTB, which encodes cystatin...
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Caveolin-1 is involved in high glucose accelerated human glomerular mesangial cell senescence

Feng X, Gao W, Li Y

BACKGROUND/AIMS:: We demonstrated the role of caveolin-1 involved in high glucose (HG)-induced glomerular mesangial cells (GMCs) senescence. METHODS:: HG was used to stimulate GMCs. The telomere lengths were analyzed by Southern...
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Carrier screening for (CGG)n repeat expansion of FMR1 gene in Korean women

Kang KM, Sung SR, Park JE, Shin YJ, Park SH, Chin MU, Lyu SW, Cha DH, Shim SH

PURPOSE: We examined the prevalence and CGG/AGG repeat structure of expanded alleles of the FMR1 gene in preconceptional and pregnant Korean women. MATERIALS AND METHODS: The CGG repeats in the FMR1...
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Clinical Validation of AdvanSure GenoBlot Assay as Primary Screening and Test of Cure for Human Papillomavirus Infection

Kahng J, Oh EJ, Lee HN, Lee DW, Kim Y

BACKGROUND: Clinical specificity and sensitivity are essential factors in the adoption of a human papillomavirus (HPV) test as a primary screening tool and test of cure after treatment of cervical...
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Elucidating the role of ApxI in hemolysis and cellular damage by using a novel apxIA mutant of Actinobacillus pleuropneumoniae serotype 10

Chang NY, Chen ZW, Chen TH, Liao JW, Lin CC, Chien MS, Lee WC, Lin JH, Hsuan SL

Exotoxins produced by Actinobacillus (A.) pleuropneumoniae (Apx) play major roles in the pathogenesis of pleuropneumonia in swine. This study investigated the role of ApxI in hemolysis and cellular damage using...
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Southern Analysis after Long-range PCR: Clinical Application in Korean Patients with Myotonic Dystrophy 1

Yum MS, Lee BH, Kim GH, Lee JJ, Choi SH, Lee JY, Kim JM, Kim YM, Ko TS, Yoo HW

PURPOSE: Myotonic dystrophy 1 (DM1, OMIM 160900) is an autosomal-dominant muscular disorder caused by an expansion of CTG repeats in the 3' UTR of the DMPK gene. Variable expansions of...
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Prenatal Population Screening for Fragile X Carrier and the Prevalence of Premutation Carriers in Korea

Han SH, Heo YA, Yang YH, Kim YJ, Cho HI, Lee KR

PURPOSE: Fragile X carrier detection before or at early pregnancy through a wide screening program may not only confer a risk of having offspring with Fragile X syndrome (FXS), but...
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A Case Report on 30-Week Premature Twin Babies with Congenital Myotonic Dystrophy Conceived by In Vitro Fertilization

Son SB, Chun JM, Kim KA, Ko SY, Lee YK, Shin SM

Congenital myotonic dystrophy type 1 (DM1) presents severe generalized weakness, hypotonia, and respiratory compromise after delivery with high mortality and poor prognosis. We presented a congenital DM1 of premature twins...
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Multilocus Sequence Typing for Candida albicans Isolates from Candidemic Patients: Comparison with Southern Blot Hybridization and Pulsed-field Gel Electrophoresis Analysis

Myoung Y, Shin JH, Lee JS, Kim SH, Shin MG, Suh SP, Ryang DW

BACKGROUND: We evaluated the efficacy of multilocus sequence typing (MLST) for assessing the genetic relationship among Candida albicans isolates from patients with candidemia in a hospital setting. METHODS: A total of...
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Detection of Prevotella intermedia and Prevotella nigrescens using Pn17 and Pn34 DNA Probes

Park CH, Kim PS, Kim HS, Min JB, Hwang HK, Jang HS, Cho KW, Baek DH, Kook JK

  • KMID: 1719928
  • Int J Oral Biol.
  • 2010 Mar;35(1):13-19.
The DNA probes Pn17 and Pn34 were evaluated for their ability to specifically detect clinical strains of P. intermedia and P. nigrescens from a Korean population by dot blot hybridization....
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Highly Efficient Electroporation-mediated Transformation into Edible Mushroom Flammulina velutipes

Kim JK, Park YJ, Kong WS, Kang HW

  • KMID: 2312600
  • Mycobiology.
  • 2010 Dec;38(4):331-335.
In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using...
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Cloning of a Family of Serine Protease Genes Expressed in German Cockroach(Blattella germanica) and Possibility as Allergens

Lee WY, Kim HB, Park JR, Park SH, Park KW, Ock MS, Yu TH

  • KMID: 2276895
  • Korean J Otorhinolaryngol-Head Neck Surg.
  • 2008 Jun;51(6):528-534.
BACKGROUND AND OBJECTIVES: The serine protease allergens have been known as one of the most important inhalent allergens of insects as well as house dust mites. There is no known...
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Molecular diagnosis of fragile X syndrome in a female child

Jeong SY, Yang JA, Kim HJ

  • KMID: 2184446
  • J Genet Med.
  • 2008 Jun;5(1):41-46.
PURPOSE: Fragile X syndrome (FXS) is the most common heritable cause of cognitive impairment. FXS is caused by hyperexpansion and hypermethylation of a polymorphic CGG trinucleotide repeat in the 5'...
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Secretion of Biologically Active Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor by Transduced Gastric Cancer Cells

Kim HY, Park GS, Shin HJ, Park S, Kim K, Kim HI

PURPOSE: Gastric cancer has the highest incidence rate among cancers in Asia. The advanced type of signet ring cell carcinoma has poor prognosis compared to other types of gastric cancer....
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Molecular and Clinical Characteristics of Myotonic Dystrophy Type 1 in Koreans

Kim SY, Kim JY, Kim GP, Sung JJ, Lim KS, Lee KW, Chae JH, Hong YH, Seong MW, Park SS

BACKGROUND: Myotonic dystrophy type 1 (DM1) is an autosomal-dominant muscular dystrophy caused by expansion of cytosine-thymine-guanine (CTG) trinucleotide repeats in the myotonic dystrophy protein kinase (DMPK) gene. The clinical features...
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Clinical and Genetic Analysis of Korean Patients with Facioscapulohumeral Muscular Dystrophy

Ki CS, Lee ST, Kim KS, Kim JW, Hong YH, Sung JJ, Park KS, Lee KW

Facioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominantly inherited muscular disorder, which is characterized by weakness of facial, shoulder and hip girdle, humeral, and anterior distal leg muscles. The FSHD...
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Role of HGF/c-Met in Serum-Starved ARPE-19 Cells

Jun EJ, Kim HS, Kim YH

PURPOSE: Hepatocyte growth factor (HGF) and its receptor (HGFR/c-Met) regulate motility, mitogenesis, and morphogenesis in a cell type-dependent fashion. We report the role of HGF and c-Met on stress-induced...
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In vivo determination of the gap2 gene promoter activity in Giardia lamblia

Yang HW, Kim J, Yong TS, Park SJ

A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase...
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Class 1 Integron Carriage in Extended-spectrum beta-lactamase-producing K. pneumoniae

Kim J

  • KMID: 1963266
  • J Bacteriol Virol.
  • 2004 Sep;34(3):181-189.
Many antibiotic resistance genes found in gram-negative clinical isolates are a part of a gene cassette inserted into an integron. Incidence of the integron carriage in extended-spectrum beta-lactamases (ESBLs)-producing clinical...
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