J Periodontal Implant Sci.  2012 Oct;42(5):151-157. 10.5051/jpis.2012.42.5.151.

Cyclooxygenase 2 gene polymorphisms and chronic periodontitis in a North Indian population: a pilot study

• Daing A
• Singh SV
• Saimbi CS
• Khan MA
• Rath SK

• KMID: 2212074
• DOI: http://doi.org/10.5051/jpis.2012.42.5.151

Abstract

PURPOSE
Cyclooxygenase (COX) enzyme catalyzes the production of prostaglandins, which are important mediators of tissue destruction in periodontitis. Single nucleotide polymorphisms of COX2 enzyme have been associated with increasing susceptibility to inflammatory diseases. The present study evaluates the association of two single nucleotide polymorphisms in COX2 gene (-1195G>A and 8473C>T) with chronic periodontitis in North Indians.
METHODS
Both SNPs and their haplotypes were used to explore the associations between COX2 polymorphisms and chronic periodontitis in 56 patients and 60 controls. Genotyping was done by polymerase chain reaction followed by restriction fragment length polymorphism. Chi-square test and logistic regression analysis were performed for association analysis.
RESULTS
By the individual genotype analysis, mutant genotypes (GA and AA) of COX2 -1195 showed more than a two fold risk (odds ratio [OR]>2) and COX2 8473 (TC and CC) showed a reduced risk for the disease, but the findings were not statistically significant. Haplotype analysis showed that the frequency of the haplotype AT was higher in the case group and a significant association was found for haplotype AT (OR, 1.79; 95% confidence interval, 1.03 to 3.11; P=0.0370) indicating an association between the AT haplotype of COX2 gene SNPs and chronic periodontitis.
CONCLUSIONS
Individual genotypes of both the SNPs were not associated while haplotype AT was found to be associated with chronic periodontitis in North Indians.

Keyword

Chronic periodontitis; Cyclooxygenase 2; Single nucleotide polymorphism

MeSH Terms

Chronic Periodontitis
Cyclooxygenase 2
Genotype
Haplotypes
Humans
Logistic Models
Periodontitis
Pilot Projects
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Prostaglandin-Endoperoxide Synthases
Prostaglandins
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Prostaglandins

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