J Periodontal Implant Sci.  2013 Oct;43(5):215-220. 10.5051/jpis.2013.43.5.215.

Determination of levels of nitric oxide in smoker and nonsmoker patients with chronic periodontitis

• Wadhwa D
• Bey A
• Hasija M
• Moin S
• Kumar A
• Aman S
• Sharma VK

• KMID: 2212029
• DOI: http://doi.org/10.5051/jpis.2013.43.5.215

Abstract

PURPOSE
Cigarette smoking is a major risk factor in periodontal diseases. The pathogenesis of periodontal diseases may be affected by alterations of the inflammatory response by smoke. Nitric oxide (NO) is a gaseous, colorless, highly reactive, short-lived free radical with a pivotal role in the regulation of various physiological and pathological mechanisms in the body. It is important in host defense and homeostasis, on the one hand, whereas, on the other hand, it modulates the inflammatory response in periodontitis, leading to harmful effects. The aim of this study was to assess the levels of NO in both the serum and saliva of smokers and nonsmokers having chronic periodontitis and to compare them with periodontally healthy controls.
METHODS
Sixty subjects participated in the study and were divided into three groups: group I, healthy nonsmoking subjects; group II, nonsmoking patients with chronic periodontitis; group III, smoking patients with chronic periodontitis. Each group consisted of twenty subjects. The biochemical estimation of NO in the collected serum and in the saliva was performed using the Griess colorimetric reaction.
RESULTS
The results showed that the mean value of the salivary and serum NO was greater in group II than in group I, and also greater in group III than in group II.
CONCLUSIONS
NO appears to play an important and rather complex role in the immuno-inflammatory process and in the remodeling and maintenance of osseous structures. It is therefore logical that modulation of this mediator has potential for the treatment of a number of inflammatory conditions including periodontal disease.

Keyword

Chronic periodontitis; Colorimetry; Nitric oxide; Smoking

MeSH Terms

Chronic Periodontitis*
Colorimetry
Homeostasis
Humans
Nitric Oxide*
Periodontal Diseases
Periodontitis
Risk Factors
Saliva
Smoke
Smoking
Nitric Oxide
Smoke

Figure

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  Figure 1 Nitric oxide estimation standard curve.

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