Effects of Human Basic Fibroblast Growth Factor (bFGF) on Autoclaved Calvarial Bone Autograft in Rat Model

Park, Jung Min; Kim, Jeong Hae; Lee, Keun Cheol; Kim, Jeong Tae; Kim, Seok Kwun; Roh, Mee Sook; Hong, Sook Hee

Abstract

This study was undertaken to investigate if the resected (4 X 4 mm), autocalved(121degrees C, 1kg/cm2, 30 minutes) and grafted calvarial bone supplemented with human basic fibroblast growth factor demonstrated better osteogenesis or bony union compared with nonsupplemented bone graft. Total 32 male Sprague-Dawely rats were used in this experiment. The subjects were divided into 4 groups. Group 1(n = 8) was autografted as control, group 2(n = 8) was autoclaved and autografted as control, group 3(n = 8) was autoclaved supplemented with bone marrow as control, group 4(n = 8) was autoclaved and supplemented with fibroblast growth factor.The grafted bone fragments were investigaed with radiologic, histologic(light and electron microscope, fluorescence microscope), histomorphometric analysis after 4 and 12 weeks.After 4 weeks, no major differences in osteogenesis (bone fill in bony defect) were observed between each group, but the degree of lacuna filling of osteocytes was significantly higher in group 4(supplemented with fibroblast growth factor) compared with group 1(autografted). After 12 weeks, there was significantly increased bone fill in bony defect in group 4(supplemented with fibroblast growth factor) compared with group 2(autoclaved) but it was significantly lower than that of group 1(autografted).Considering this results, autoclaved bone can be revitalized gradually by invading new bone and this procedure is enhanced by basic fibroblast growth factor. Calvarial bone grafts supplemented with basic fibroblast growth factor begins to make new bone earlier than any other control groups.