J Korean Orthop Res Soc.
1998 Apr;1(1):14-22.
In Vivo Transfection of Rabbit Synovium using the Particle Bombardment Method
- Affiliations
-
- 1Department of Orthopaedic Surgery, College of Medicine, Seoul National University, Seoul, Korea.
Abstract
- Treatment of arthritis with the gene-transfering technique is one of tile important applications of gene therapy in orthopedics. The synovium can be transfected with the genes producing therapeutic proteins. Among the numerous methods of transfering genes, particle bombardment or gene gun is one of the most versatile tools and has the advantage of easy accessibility and efficiency. The authors planed to transfer the reporter gene into the synovium in vivo and confirm the expression of the gene. Cold particle microcarriers to deliver the DNA were coated with plasmids containing human placental alkali no phosphatase cDNA as a marker gene. The microcarriers were projected with the aid of the hand-held gene gun system into the rabbit knee synovium. After three days, the synovium was harvested and frozen sections of the tissue were made. The expression of the reporter gene in the synovium was confirmed with alkaline phoshatase staining. In summary, the authors have shown that the reporter gene was successfully delivered into the rabbit knee synovium in vivo with particle bombardment method and transient expression of the gene was confirmed with special staining. Our study suggest that in vivo transfection of the synovium using tile gene gun can be applied as a novel method for treating arthritis.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Production of TGF-beta Transfected Fibroblast and Myoblast Stable Cell Lines and their Viability in Rabbit Achilles Tendon
- In Vivo reaction of the Highly Porous Glass Ceramics in the Rabbit Tibia: Radiological and Histological Analysis
- Transfection of SV 40 Large T Antigen into Corneal Endothelial Cells
- Morphology and Adhesion Complex of Cultured Epithelium, on Amniotic Membrane in Vitro and in Vivo
- Cytokine Response of the Synovium-like Membrane to the Particulate Biomaterial in Murine Air-Pouch Model
