J Korean Soc Virol.  1999 Jun;29(2):99-105.

Detection of Glycoproteins (B and D) and Ttymidine Kinase Genes of Herpes simplex virus Type 2 Strain G

• Kang H
• Park JK
• Uh HS
• Kim SY
• Lee HH

Abstract

BamHI restriction patters and genomic library of Herpes simplex virus type 2 (HSV-2) stram G were constructed, and locations of the glycoproteins gB and gD, and it genes on the fragments were detected by Southern blot analysis. HISV-2 genomic DNAs were cleaved into twenty-seven fragments by BamHI enzyme in the range of 0.72 to 15.08 (total 150.44 kb), which were cloned into the BamHI site of pBluescript SK(+) to construct genome library of the HSV-2. The library was named by the order of the fragment size from smallest one to largest one. HSV-2 glycoprotein gD gene was located in PHLA2-21 and PHLA2-22 recombinant plasmids, gB gene in PHLA2-24 plasmic, and it gene in PHLA2-11 clone by Southern blot analysis.

Keyword

Herpes simplex virus; Glycoprotein; Glycoproteins B and D genes; Thymidine kinase gene; TK protein

MeSH Terms

Blotting, Southern
Clone Cells
DNA
Genomic Library
Glycoproteins*
Herpes Simplex*
Herpesvirus 2, Human*
Phosphotransferases*
Plasmids
Simplexvirus*
DNA
Glycoproteins
Phosphotransferases