Korean J Hepatobiliary Pancreat Surg.  2001 Jul;5(1):19-25.

Hepatic Ischemia-Reperfusion Injury induced by Continuous and Intermittent Inflow Occlusion in Rats

• Cho NC
• Won DY
• Kim MS
• Cho MY
• Yoon KJ
• Kim JS
• Kim IY
• Kim DS
• Rhoe BS

Abstract

BACKGROUND/AIMS: Recent studies suggest possibility of continuous and prolonged liver ischemia exceeding one hour. We compared mortality rates, liver function, serum Interleukin-6(IL-6) concentration and liver cell necrosis after continuous and intermittent hepatic ischemia in rats.
METHODS
Sixty rats were divided into 6 groups to compare 7 day mortality rate. Continuous and intermittent left hepatic inflow occlusion was performed for a total period of 45, 60 and 90 minutes. In a separate study, following 90 minutes continuous or intermittent ischemia, systemic blood was sampled at 0 minute, 6 hours and 24 hours after final clamp release for measurement of SGOT, SGPT and IL-6. Pathologic examination was performed 24 hours or 7 days after reperfusion accordingly.
RESULTS
There were no differences in the mortality rates within seven days. There were no differences in the level of SGOT, SGPT and IL-6 between each experimental group. In a pathologic examination, similar liver cell necrosis was found in each group until 24 hours of reperfusion. However, at 7 days after reperfusion, significantly higher grade of hepatic necrosis was noted in the group having continuous ischemia compared with intermittent ischemia of 90 minutes(p<0.05).
CONCLUSION
Continuous ischemia is associated with significant risk in the aspect of pathologic study, although it did not affect short term mortality rates.

Keyword

Hepatic ischemia; continuous or intermittent occlusion

MeSH Terms

Alanine Transaminase
Animals
Aspartate Aminotransferases
Interleukin-6
Ischemia
Liver
Mortality
Necrosis
Rats*
Reperfusion
Reperfusion Injury*
Alanine Transaminase
Aspartate Aminotransferases
Interleukin-6