Genomics Inform.  2011 Dec;9(4):161-172.

Gene Expression Analysis of Galpha13-/- Knockout Mouse Embryos Reveals Perturbations in Galpha13 Signaling Related to Angiogenesis and Hypoxia

• Park JH
• Choi S

Abstract

Angiogenesis is regulated by a large number of molecules and complex signaling mechanisms. The G protein Galpha13 is a part of this signaling mechanism as an endothelial cell movement regulator. Gene expression analysis of Galpha13 knockout mouse embryos was carried out to identify the role of Galpha13 in angiogenesis signaling during embryonic development. Hypoxia-inducible response factors including those acting as regulators of angiogenesis were over expressed, while genes related to the cell cycle, DNA replication, protein modification and cell-cell dissociation were under expressed. Functional annotation and network analysis indicate that Galpha13 -/- embryonic mice were exposed to hypoxic conditions. The present analysis of the time course highlighted the significantly high levels of disorder in the development of the cardiovascular system. The data suggested that hypoxia-inducible factors including those associated with angiogenesis and abnormalities related to endothelial cell division contributed to the developmental failure of Galpha13 knockout mouse embryos.

Keyword

angiogenesis; gene expression; G protein Galpha13; G protein signaling; hypoxia

MeSH Terms

Animals
Anoxia
Cardiovascular System
Cell Cycle
Dissociative Disorders
DNA Replication
Embryonic Development
Embryonic Structures
Endothelial Cells
Female
Gene Expression
GTP-Binding Proteins
Mice
Mice, Knockout
Pregnancy
GTP-Binding Proteins