Korean J Urol.  2007 Nov;48(11):1131-1138. 10.4111/kju.2007.48.11.1131.

The Effect of Stress on Bladder Stability

• Kim KH
• Yoon H

• KMID: 1997089
• DOI: http://doi.org/10.4111/kju.2007.48.11.1131

Abstract

PURPOSE: This study was designed to examine the effect of stress on the pathophysiology of bladder stability via the Rho-kinase and nitric oxide synthase activity, which are required for muscle contraction and relaxation within the bladder.
MATERIALS AND METHODS
Animal testing was conducted in two separate sessions. In the first experiment, 36 female Sprague-Dawley rats weighing about 230-270g each were employed. 18 rats were placed in the control group and 18 rats were placed in the test group. The second testing was conducted using metabolic cages. Six rats were placed in the control group and six rats were placed in the test group under a stressful environment.
RESULTS
The results showed that the frequency of urination was significantly increased with time in the test group(p<0.05). However, the volume of voided urine decreased, thereby suggesting stress was a cause of overactive bladder. Analysis of bladder tissue for nitric oxide synthase (NOS) and RhoA-binding kinase(ROKalpha), important components of contraction and relaxation of bladder muscle, revealed that the levels of iNOS and ROKalpha were significantly increased with continued application of stress. This suggests that stress affects the levels of NOS and ROKalpha in an overactive bladder to influence contraction and relaxation of the bladder muscle.
CONCLUSIONS
An increase in the ROKalpha expression and changes of the NOS expressions in the rats with chronic stress pathophysiologicaly support that stress contributes to the clinical symptoms of overactive bladder.

Keyword

Stress; Urinary bladder; Overactive

MeSH Terms

Animals
Female
Humans
Muscle Contraction
Nitric Oxide Synthase
Rats
Rats, Sprague-Dawley
Relaxation
rho-Associated Kinases
Urinary Bladder*
Urinary Bladder, Overactive
Urination
Nitric Oxide Synthase
rho-Associated Kinases

Figure

• 

  Fig. 1 Effect of stress on the urinary bladder function test parameters. (A) Voiding frequency: This figure shows the total frequency of voiding within 3 hours, (B) Voiding volume: These graphs presented volume (µl)/void in the stress and control groups with prolonged application of the stress (*: p<0.05, †: p<0.01).

• 

  Fig. 2 Pattern of the DAB positive area in bladder tisssue after immunohistochemical staining for nNOS, iNOS and ROKα at 20 days. Magnification 200x (C: urothelium, D: submucosa, E: muscularis mucosa) DAB: diamino benzidine, nNOS: neuronal nitric oxide synthase, iNOS: inducible nitric oxide synthase, ROKα: rhoA-binding kinase.

• 

  Fig. 3 Western blotting assay of bladder tissue for determining the protein expression level (*: p<0.05). (A) Western blotting assay for nNOS expression. (B) Western blotting assay for iNOS expression. (C) Western blotting assay for ROKα expression (nNOS: neuronal nitric oxide synthase, iNOS: inducible nitric oxide synthase, ROKα: rhoA-binding kinase).

• 

  Fig. 4 RT-PCR analysis of bladder tissue for determining the expression level of the mRNA of ROKα transcript (*: p<0.05) RT-PCR: reverse trnascript-polymerase chain reaction, ROKα: rhoA-binding kinase.

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