Korean J Vet Res.
2013 Mar;53(1):25-28.
Application of SYBR Green real-time PCR assay for the specific detection of Salmonella spp
- Affiliations
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- 1Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea. yoohs@snu.ac.kr
- 2Bacterial Disease Division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-757, Korea.
Abstract
- The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle (C(T)) of Salmonella spp. was 11.83 +/- 0.78 while non-Salmonella spp. was 30.86 +/- 1.19. Correlation coefficients of standard curves constructed using C(T) versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity (R2 = 0.993; slope = 3.563). Minimum level of detection with the method was > 10(2) colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.
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