J Korean Neurol Assoc.
1992 Sep;10(3):288-297.
Effects of Experimental Ischemia on Parvalbumin-and Calbindin-Immunoreactive Elements in the Rabbit Spinal Cord
- Affiliations
-
- 1Department of Neurology, College of Medicine, Chonnam University.
- 2Department of Pharmacology, College of Medicine, Chonnam University.
- 3Department of Anatomy, College of Medicine, Chonnam University.
Abstract
- To understamd the changes in parvalbumm- and calbindin-immunoreactive neurons and fibers during the spinal cord ischemia, expression of two kinds of calcium bindlng protein, parvalbumin (PV) and calbindin D-28K (calbindin), immunocytochemically an activities of cytochrome oxidase (CO) and acetylcholinesterase (AChE), histochemically global ischemic spinal cord of the rabbit were investigated under thiopental sodium anesthesia, adult rabbits(2,0-2.5 Kg) was cannulated through the left femoral artery with 4F Berman amgiographic catheter. Which was advanced 15 so that tbe tip of the catheter was in the ahdominal aorta just below the orign of the renal artery. After the rabbits were recovered from anesthesia, the balloon was inflated induce ischemia for15 minutes with recording abdominal aortic blood pressure. At 48 hours after reperfusion, the rabbits were fixed with transcardiac perfusion. In the sham-operated controls, the rabbit was treated same procedure except inflation of the ball. In tbe ischemic spinal cord, CO activitieS were generally decreased in the gray matter, especially in laminae III-V and AChE activities were slightly decreased in laminae X amd III-IV. The degree of immunoreactivities for PV and calbindin was decreased in whole gray matter of ischemic cord. In laminae II-III of the ischemic lumbar cord, number of PV amd calbindin-immunoreactive neurons was signilicantly decreased and vacuolations were observed. In laminae V-IX amd X of ischemic lumbar cord. The numbe of jmmunoreactive neurons was not changed, but degree of immunoreactivities for and calbindin was decreased. Thses findings suggest that PV and calbindin-immunoreactive neurons in laminae II-III are more vulnerable than other neurons to ischemic injury, and PV and calbindin may have some roles in hypoxic neuronal injury.
MeSH Terms
-
Acetylcholinesterase
Adult
Anesthesia
Aorta
Arterial Pressure
Calbindins
Calcium
Catheters
Electron Transport Complex IV
Femoral Artery
Humans
Inflation, Economic
Ischemia*
Neurons
Perfusion
Rabbits
Renal Artery
Reperfusion
Spinal Cord Ischemia
Spinal Cord*
Thiopental
Acetylcholinesterase
Calcium
Electron Transport Complex IV
Thiopental
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