Korean J Anat.
2006 Apr;39(2):91-102.
Screening of Low Molecular Metabolite, FS390 as an Inhibitor of Neurotransmitter Release from PC12 Cells
- Affiliations
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- 1Department of Biology, College of Natural Science, Chonbuk National University, Jeonju 561-756, Korea.
- 2Department of Anatomy, College of Medicine, Wonkwang University, Iksan 570-749, Korea.
- 3Department of Infection Biology, College of Medicine, Wonkwang University, Iksan 570-749, Korea. yusikmj@gmail.com
Abstract
- We established an in vitro experimental system using the following procedure. We first introduced tritium-labeled norepinephrine ([3H]-NE) into PC12 cells. The [3H]-NE incorporated-PC12 cells were stimulated by a high concentration (60 mM) of K+ buffer during 12 minutes. Then, we collected 100 microliter supernatant and counted the amount of [3H]-NE release from PC12 cells with a scintillation counter. After screening fungal, Streptomyces spp. or bacterial product using this experimental sytem, we obtained FS390 from Streptomyces spp. which inhibited [3H]-NE release from PC12 cells. FS390 also inhibits the release of ATP as a neurotransmitter of PC12 cells and rat cortical neurons. The inhibitory effect was seen even when the PC12 cells were treated with low K+ buffer containing ionomycin (1 micrometer) as an ionopore. This result suggests that the inhibitory action of FS390 on neurotransmitter release appeared after the influx of Ca2+.
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