J Lab Med Qual Assur.  2004 Jun;26(1):211-214.

Comparision between Polymerase Chain Reaction and QuickVue(TM) EIA method in Diagnosis of Chlamydia trachomatis Infection

• Lee CH
• Kim SJ
• Yun BI

Abstract

BACKGROUND: Chlamydia trachomatis is one of the most common causative agents of the sexual transmitted diseases, and the accurate diagnosis and early treatment are very important to prevent the transmission. So, we evaluated the polymerase chain reaction (PCR) and QuickVue(TM) (Quidel Corp. San Diego, CA) EIA method as a routine clinical laboratory test.
METHODS
The 80 cervical swab samples including pelvic inflammatory disease, cervicitis and tube-ovarian abscess were tested using PCR with plasmid specific primers T1, T2 and commercially available QuickVue(TM) EIA kit.
RESULTS
The positivities of the PCR and QuickVue(TM) EIA test were 17.5% (14/80 samples) and 12.5% (10/80 samples), respectively. There were 95% (76/80 samples) of positive or negative result concordance rates and 5% (4/80 samples) of disconcordance between the two methods, in which showed PCR positive and EIA negative results.
CONCLUSIONS
We assume that PCR and QuickVue(TM) method are highly confident as a routine clinical laboratory diagnostic test for C. trachomatis infection. But more careful interpretation of QuickVue(TM) EIA and the additional study for variable samples will be needed.

Keyword

PCR; QuickVue(TM) EIA; Chlamydia trachomatis

MeSH Terms

Abscess
Chlamydia trachomatis*
Chlamydia*
Diagnosis*
Diagnostic Tests, Routine
Female
Pelvic Inflammatory Disease
Plasmids
Polymerase Chain Reaction*
Uterine Cervicitis