J Korean Assoc Oral Maxillofac Surg.  2007 Dec;33(6):583-590.

Anticancer effects of caesalpinia sappan extracts on oral carcinoma and osteosarcoma cells

Affiliations
  • 1Department of Oral Maxillofacial Surgery, School of Dentistry, Kyung Hee University, Korea. kyukab@khu.ac.kr
  • 2Department of Oral Biochemistry, School of Dentistry and Institute of Oral Biology, KyungHee University, Korea.

Abstract

Anticancer effect of methanol extract of Caesalpinia sappan L. on oral carcinoma (KB) and osteosarcoma (HOS) cells were investigated in this study. In order to elucidate the anticancer mechanism of Caesalpinia sappan L, we analyzed telomerase inhibitory effect of the methanol extract of Caesalpinia sappan L. In addition, we prepared 5 fraction samples according to its polarity differences and analyzed anticancer effects on oral carcinoma and osteosarcoma cells. Following results are obtained in this study. 1. 50percent cell proliferation inhibitory value (IC(50)) of the methanol extract of Caesalpinia sappan L. against oral carcinoma (KB) cells and osteosarcoma (HOS) cells were 9.0 microgram/ml and 10.9 microgram/ml, respectively. 2. The methanol extract of Caesalpinia sappan L. showed inhibitory effect of telomerase which is required for cancer cell immortality. Therefore, it seems that the anticancer effect of methanol extract of Caesalpinia sappan is at least partially due to telomerase inhibitory effect. 3. Five fraction samples were prepared according to its polarity and 88.7percent of ingredient of total methanol extract was transferred to ethylacetate fraction. Thin layer chromatography analysis showed that dichloromethane fraction contained ingredient with relatively high polarity and ethylacetate fraction contained similar ingredient found in total methanol extract. 4. Anticancer effect was observed in n-hexane, dichloromethane, and ethylacetate fractions. The highest anticancer effect was found in dichloromethane fraction which had IC(50) value of 4.4 microgram/ml and > 4.0 microgram/ml against oral carcinoma (KB) cells and osteosarcoma (HOS) cells, respectively.

Keyword

Caesalpinia sappan extract; Oral carcinoma; Psteosarcoma; Telomerase

MeSH Terms

Caesalpinia*
Cell Proliferation
Chromatography, Thin Layer
Methanol
Methylene Chloride
Osteosarcoma*
Telomerase
Methanol
Methylene Chloride
Telomerase
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