J Vet Sci.  2014 Jun;15(2):209-216. 10.4142/jvs.2014.15.2.209.

Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus

• Lv L
• Li X
• Liu G
• Li R
• Liu Q
• Shen H
• Wang W
• Xue C
• Cao Y

• KMID: 1784641
• DOI: http://doi.org/10.4142/jvs.2014.15.2.209

Abstract

Infectious bronchitis virus (IBV) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. Vaccination is the most effective method of preventing infection and controlling the spread of IBV, but currently available inactivated and attenuated virus vaccines have some disadvantages. We developed a chimeric virus-like particle (VLP)-based candidate vaccine for IBV protection. The chimeric VLP was composed of matrix 1 protein from avian influenza H5N1 virus and a fusion protein neuraminidase (NA)/spike 1 (S1) that was generated by fusing IBV S1 protein to the cytoplasmic and transmembrane domains of NA protein of avian influenza H5N1 virus. The chimeric VLPs elicited significantly higher S1-specific antibody responses in intramuscularly immunized mice and chickens than inactivated IBV viruses. Furthermore, the chimeric VLPs induced significantly higher neutralization antibody levels than inactivated H120 virus in SPF chickens. Finally, the chimeric VLPs induced significantly higher IL-4 production in mice. These results demonstrate that chimeric VLPs have the potential for use in vaccines against IBV infection.

Keyword

chimeric virus-like particle; immune response; infectious bronchitis virus

MeSH Terms

Animals
Antibodies, Viral/blood
*Chickens
Chimera/genetics/immunology
Coronavirus Infections/prevention & control/*veterinary/virology
Female
*Immunity, Innate
Infectious bronchitis virus/genetics/*immunology
Influenza A Virus, H5N1 Subtype/genetics/immunology
Injections, Intramuscular/veterinary
Mice
Mice, Inbred BALB C
Neuraminidase/genetics
Poultry Diseases/*prevention & control/virology
Recombinant Fusion Proteins/genetics/immunology
Spike Glycoprotein, Coronavirus/genetics/*immunology
Vaccines, Synthetic/administration & dosage/genetics/immunology
Vaccines, Virus-Like Particle/administration & dosage/genetics/*immunology
Viral Proteins/genetics
Antibodies, Viral
Recombinant Fusion Proteins
Neuraminidase
Spike Glycoprotein, Coronavirus
Vaccines, Synthetic
Vaccines, Virus-Like Particle
Viral Proteins