Korean J Lab Med.  2010 Dec;30(6):616-623. 10.3343/kjlm.2010.30.6.616.

Frequency of Extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase Genes in Escherichia coli and Klebsiella pneumoniae over a Three-year Period in a University Hospital in Korea

• Li XM
• Jang SJ
• Bae IK
• Park G
• Kim YS
• Shin JH
• Moon DS
• Park YJ

• KMID: 1781668
• DOI: http://doi.org/10.3343/kjlm.2010.30.6.616

Abstract

BACKGROUND
The aim of this study was to determine the yearly prevalence and genotype distribution of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae collected over a 3-yr period in Gwangju, Korea.
METHODS
Clinical isolates of E. coli and K. pneumoniae collected at Chosun University Hospital from September 15, 2005 to September 14, 2008 were evaluated. Antimicrobial susceptibility testing was performed using the Vitek II system (bioMerieux, USA) and agar dilution methods. Screening for ESBL and AmpC beta-lactamase genes was performed using PCR amplification of plasmid DNA followed by direct sequencing of the PCR products.
RESULTS
The percentage of ESBL-producing isolates was 12.6% (196/1,550) for E. coli and 26.2% (294/1,121) for K. pneumoniae. The ESBL gene sequencing results showed that the most prevalent ESBL types were CTX-M (93.5%) and SHV (12.9%) in E. coli, and SHV (73.2%) and CTX-M (46.3%) in K. pneumoniae. The most common ESBL in E. coli was CTX-M-15-like, followed by CTX-M-14-like, SHV-2a-like, and SHV-12-like. The most prevalent ESBL type in K. pneumoniae was SHV-12, followed by CTX-M-14-like and CTX-M-15-like. Fifty-one percent (21/41) of ESBL-producing K. pneumoniae with ESBL types verified by sequencing also had DHA-1-like AmpC beta-lactamases. However, none of the ESBL-producing E. coli was positive in the AmpC beta-lactamase PCR analysis.
CONCLUSIONS
In this study, the most common types of class A ESBLs identified were CTX-M-15-like in E. coli and SHV-12-like in K. pneumoniae.

Keyword

beta-lactamase; Prevalence; Escherichia coli; Klebsiella pneumonia

MeSH Terms

Bacterial Proteins/*genetics
Drug Resistance, Bacterial/genetics
Escherichia coli/*genetics/isolation & purification
Gene Frequency
Genotype
Hospitals, University
Humans
Klebsiella pneumoniae/*genetics/isolation & purification
Microbial Sensitivity Tests
Republic of Korea
Sequence Analysis, DNA
beta-Lactamases/*genetics

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